The effect of sodium hypochlorite (NaClO) on nucleic acids (NAs) was investigated. The effect of biomolecular structure on resistance to hypochlorite was analysed: plasmid bacterial DNA, calf thymus DNA, synthetic polyadenylic-uridic acid samples were studied, as well as individual nucleotides (adenosine-5’-tetraphosphoric acid and guanosin-2’, 3-cyclophosphoric acid). The effect of sodium hypochlorite on DNA was investigated depending on the concentrations of the components. We have also performed detailed analysis of the kinetics of the reaction between the NAs and NaClO. It was found that both the destruction of the secondary structure of DNA (denaturation) and the chemical modification of nitrogenous bases, presumably chlorination, occur. Presence of a stable double-stranded structure of DNA slows down the chemical reaction of sodium hypochlorite with nitrogenous bases of DNA.

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The effect of sodium hypochlorite (NaClO) on nucleic acids (NAs) was investigated depending on the concentration of the NaClO. We have performed detailed analysis of the FTIR and UV spectra of the NAs incubated with NaClO. It was found that both the destruction of the secondary structure of DNA (denaturation) and the chemical modification of nitrogenous bases occur.

The structural organization of DNA in complex with linker histone H1 and non-histone chromosomal protein HMGB1 in presence of calcium and manganese ions have been studied using FTIR and UV circular dichroism spectroscopy. We have demonstrated that the presence of calcium ions leads to the formation of highly ordered DNA-H1-HMGB1 structures, while manganese ions decrease the order in the earlier reported nanoscale complexes.