The aim: To evaluate the levels of plasminogen activator type 1 inhibitor, asymmetric dimethylarginine and endothelial nitric oxide synthase on day 10-14 in patients, depending on the presence or absence of concomitant type 2 diabetes and the type of reperfusion therapy. Materials and methods: The study involved 130 patients with acute myocardial infarction, divided into 2 groups: Group 1 consisted of patients with acute myocardial infarction with type 2 diabetes mellitus (n = 73), Group 2 comprised patients with acute type 2 diabetes mellitus (n = 57). The quantitative content of IAP-1 was determined by enzymelinked immunosorbent assay using a commercial test system manufactured by Technoclone PAI-1 ELISA Kit (Austria), NOS – Enzyme-Linked Immunosorbent Assay (ELISA) Kit for Nitric Oxide Synthase Endothelial (NOS) ADMA ELISA Kit (Austria). Results and conclusions: Percutaneous coronary intervention contributes to a significant reduction in the content of ADMA, which is a marker of endothelial dysfunction and increase NOS on the 10-14th day of acute myocardial infarction compared with standard therapy. During PCI, the level of IAP-1 did not significantly change in the time course of treatment due to post-inflammatory and post-traumatic activation of platelets in the vascular wall.

The aim of study is to evaluate the levels of endothelium-dependent mediators: endothelial nitric-oxide synthase (NOS), plasminogen activator inhibitor-1 (PAI-1) and circulating soluble CD40 ligand (sCD40L) in patients with acute myocardial infarction (AMI) and concomitant type 2 diabetes mellitus (DM). The study included 255 patients with AMI, who were divided into two groups depending on the presence of concomitant type 2 DM: 1 group — 143 patients with concomitant type 2 DM; 2 group — 112 patients without concomitant disturbances of carbohydrate metabolism. Studied endothelial-dependent indicators were investigated using enzyme-linked immunosorbent assay. Statistical data were processed using the Mann–Whitney U-test, quantitative variables were described by the following parameters: median (Me), 25th and 75th percentiles (Q1; Q3). Analyzing the studied indicators on admission of patients to the hospital, a statistically significant decrease in NOS levels (p < 0,01), as well as an increase in PAI-1 (p < 0,01) and sCD40L (p < 0,01) in the cohort of patients with AMI and concomitant type 2 DM compared with patients without disturbances of carbohydrate metabolism. This indicates a more significant violation of endothelium-dependent vasodilation, thrombin fibrinolysis and activation of intravascular inflammation caused by comorbidity. Over the next 10 days, an increase in NOS levels, a decrease in PAI-1 and sCD40L levels were observed in patients of both groups, indicating a gradual improvement of the endothelial function. However, in patients with AMI and concomitant type 2 DM, the levels of the studied endothelium-dependent mediators continued to differ statistically even on the 10th day after acute occlusion of the coronary artery. In our opinion, this tendency is caused by the negative impact of metabolicdisorders associated with type 2 DM on the endothelium of the coronary arteries in patients with insulin resistance and, apparently, may increase the risk of complications of AMI.

The aim of the study was to investigate the structural and functional changes in the male genitourinary system in the development of cryptosporidiosis.Material and methods. The study was based on biopsies and necropsies, based on histological methods.Results. The article is devoted to the study of structural and functional changes in the male genitourinary system in the development of cryptosporidiosis. The authors of the study emphasize the features of the nomenclature profile of cryptosporidiosis, its low certainty, the place among modern protozoal infections. The paper draws attention to the emergence of the disease, the specifics of its clinical course, the characteristics of the most accessible and effective methodological (histological) algorithm. The publication presents data on structural and functional changes of the urethra, testicles, kidneys of children with cryptosporidiosis (proven participation of the inflammatory component, destructive processes, trophic disorders). Researchers have focused on the peculiarities of the structure of the mucous membranes of the genitourinary organs in terms of cryptosporidiosis, destruction of the epithelium, microcirculation disorders. It is assumed that the factors of influence / strengthening of the latter were the activation of lysosomal (hydrolytic) enzymes capable of cleaving lipid - protein complexes of membranes.As a result, the opinion is expressed about the role of cryptosporidiosis in the development of male infertility.Conclusions. In the case of cryptosporidiosis, the most vulnerable structures of the male genitourinary system are cells of mesenchymal origin (epithelium).They are characterized by atrophy, desquamation, destructive-degenerative changes, necrosis. The destruction of the endothelial layer of microvessels leads to defects in the layers of the walls of the latter, thrombosis, the development of stasis, impaired microcirculation and trophism

The work is a piece of the study of the department of internal medicine № 2 and cllinical immunology and allergology of Kharkiv National Medical University «Profibrotic and immunoinflammatory factors and anemic syndrome as markers of prognosis of CHF in patients with coronary heart disease and type 2 diabetes mellitus within cardiorenal continuum» (State registration number: 0114U003389).Institution, which financed the research: Ministry of Health of Ukraine. The authors assume responsibility for the published work. The authors guarantee absence of competing interests and their own financial interest when carrying out the research and writing the article.The manuscript was received by the editorial staff 29.10.2018.