Calluna vulgaris L. Hull (Ericaceae) has been used for treatment of urinary tract infections in traditional medicine. In this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and ranged between 67.55 to 142.46 mg GAE/g. The concentrations of flavonoids in extracts were determined using spectrophotometric method with aluminum chloride and the values ranged from 42.11 to 63.68 mg RUE/g. The aqueous extract of C. vulgaris showed a significant antibacterial activity. The values of MIC were in the range from 2.5 mg/ml to 20 mg/ml for this extract. Proteus vulgaris strains were found to be the most sensitive. The results obtained suggest that all tested extracts of C. vulgaris inhibit the growth of human pathogens, especially the aqueous extract.

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of this preparation exhibited the most potent inhibitory activity, with an IC 50 value of 1.613 mg/ml, followed by the ethanolic extract of Ligusticum sinense (Kot hua bua) (IC 50 = 3.769 mg/ml) and Nigella sativa (Thian dam) (IC 50 = 4.085 mg/ml). The water and ethanol-water extracts of all plants were apparently inactive (IC 50 > 100 mg/ml). These results can support using Prasaprohyai in Thai traditional medicine for antipyretic caused by inflammation. References: [1] Foundation of resuscitate and encourage