Calluna vulgaris L. Hull (Ericaceae) has been used for treatment of urinary tract infections in traditional medicine. In this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and ranged between 67.55 to 142.46 mg GAE/g. The concentrations of flavonoids in extracts were determined using spectrophotometric method with aluminum chloride and the values ranged from 42.11 to 63.68 mg RUE/g. The aqueous extract of C. vulgaris showed a significant antibacterial activity. The values of MIC were in the range from 2.5 mg/ml to 20 mg/ml for this extract. Proteus vulgaris strains were found to be the most sensitive. The results obtained suggest that all tested extracts of C. vulgaris inhibit the growth of human pathogens, especially the aqueous extract.

Cannabinoids are typical chemical ingredients of a Cannabis sativa L. plant. The main psychoactive ingredient of this plant is Δ 9-tetrahydrocannabinol, whose content mostly determines the strength or potential of a cannabis product. In addition to this ingredient, the forensic analysis also includes determination of the presence and content of cannabidiol and cannabinol. Over the last couple of decades, there have been changes in chemical composition of cannabis products in many countries, and they manifest through the increase of total psychoactive potential. Monitoring of basic cannabinoids content and their mutual ratios, processed in this paper, has shown that there is a growing number of samples with high tetrahydrocannabinol (THC) content, while the cannabidiol (CBD) content is very low or is not present in samples at all. The THC/CBD ratio also confirms changes in plant chemotype in terms of increasing total psychoactive potential of a cannabis plant.

The title compounds were prepared by condensation of N-chlorocarbonyl-N-phenyl-4-aminobenzo-(-pyrone with diethylamine dibutylamine and isopropylamine. The starting compound used for condensation with amines was obtained by a reaction of N-phenyl-4-aminobenzo-(-pyrone with oxalyl chloride

The preparation of biologically active material is one of the most interesting trends in the chemical modification of cellulose. The possibility of obtaining biologically active cellulosic material by sorption of procaine hydrochloride and gentamicin sulphate on CMC was investigated in this paper. The sorption of therapeutics on CMC samples with different degree of substitution was carried out in water solutions of anesthetic and antibiotic, while the desorption was done in 0.95% NaCl solution. The amount of bonded and released therapeutics was determined by UV spe-ctrophotometry. It was found that degree of substitution of car-boxymethylcellulose and concentration of chemotherapeutic water solution have significant influence on the amount of bonded anesthetic and antibiotic. The maximum amount of bonded chemotherapeutic was 72.15 mg of procaine hydrochloride and 165.49 mg of gentamicin sulphate per gram CMC. In both cases the amounts of bonded chemotherape-utics correspond to the concentration of local anesthetic and antibiotic preparation that are used in therapeutic purposes

ABSTRACT. The products of the Cannabis sativa L. plant are the most widely used type of illegal drugs. In addition to growing under natural conditions, illegal breeders tend to produce, under artificial conditions, a plant with a high psychoactive potential i.e. high content of the psychoactive ingredient tetrahydrocannabinol. This paper includes the analyses results of the content of tetrahydrocannabinol in cannabis plant samples seized in the Republic of Srpska from 1999 to 2008 and from 2011 to 2016, in order to monitor the psychoactive potential of the samples available on the illegal market and the assessment trends in the future. The analyses of tetrahydrocannabinol content conducted for 3.718 samples was performed by gas chromatography with flame ionization detector according to the method of external standard. The results showed that the illegal market has samples of cannabis plant material with a noticeable difference in psychoactive ingredient content, with an increase in the number of high-potent samples. Besides the high-potent samples, health risks are caused by a great variety and sudden changes in potential of illegal drug samples, as it is necessary to continue to monitor the psychoactive potential of cannabis plants samples on the illegal market.