Summary. -The generation of antiviral drugs from herbs and other natural resources with traditionally longconfirmed effects is an efficient approach. So far, no herb or components from herbs that could inhibit bovine herpesvirus type 1 (BoHV-1) replication have been described. In this study, the antiviral effect of curcumin, a natural phenolic constituent of the spice turmeric, on BoHV-1 replication was evaluated in cell culture. We demonstrated that curcumin impairs BoHV-1 viral particles and affects the virus post-binding entry process. Furthermore, curcumin upregulated the proportion of the plasma membrane adopting a lipid raft conformation in MDBK cells, which supported the previous reports that curcumin can modulate the lipid bilayer. Though the antiviral mechanism of curcumin on BoHV-1 needs further study, we identified for the first time a component from herb that could inhibit BoHV-1 replication, in vitro.Keywords: BoHV-1; curcumin; lipid raft; virus entry * Corresponding author. E-mail: yzgqzhu@yzu.edu.cn; phone: +86-514-87311374. Abbreviations: BoHV-1 = bovine herpesvirus type 1; CTB-FITC = fluorescent cholera toxin B subunit; DMSO = dimethyl sulfoxide; HSV = herpes simplex virus; MβCD = methyl-β-cyclodextrin; MDBK = Madin-Darby bovine kidney; MOI = multiplicity of infection; MTT = 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; p.i. = post infection; TCID 50 = tissue culture infection dose 50%