Beet necrotic yellow vein virus coat protein (BNYVV CP) gene cloned from BNYVV in Inner Mongolia of China was transferred to sugar beet explants using agrobacterium tumefaciens-mediated method. Transgenic regeneration shoots were induced from petioles and cotyledons tissues of sugar beet. Three sugar beet lines that were fit for genetic transformation experiment were screened, which were Sumfull8-1, Sumfull8-3 and 9718. The experiment results showed that plant regeneration frequency was influenced by many factors, which include hormone compositions and concentration in shoots regeneration medium, culture conditions, sugar beet genotype, original explant types and culture methods. Influence factors of transgenic shoot regeneration frequency for sugar beet explants were discussed in this paper. Efficient genetic transformation system and transgenic shoots regeneration system of sugar beet were established.

Temperature affected ethanol fermentation of energy beet greatly.This study showed that yeast HADY was tolerant to high temperature and when the fermentation temperature was 45°C,the translation rate of ethanol was higher.The three main factors were screened out from nine factors related fermentation by Plackett-Burman design and response surface methodology, such as substrate concentration, ratio of solvent to material, added HADY amount,nutrient salts, phosphorus addition amount, pH value, rotational speed, fermentation temperature and fermentation time. The three main factors included substrate concentration, fermentation temperature and phosphorus addition amount.The optimized technological parameters of HADY were as follows:substrate concentration12%, ratio of solvent to material 1:1, added HADY15%,nutrient salts 0.5,added phosphorus 0.9%, pH 5.0,rotation speed 130r/min, fermentation temperature 37°C and fermentation time 44 hours.At the time, the result showed that the translation rate of ethanol was 89.43%.

In order to meet the needs of industrial mass production,people began to explore the effect on different modes to ethanol fermentation.Ethanol fermentation with energy beet was done by using batch fermentation and continuous fermentation.Results showed that batch fermentation was better than continuous mode,but its technology was cockamamie.The residual glucose content in fermenting mash was high when fermentation was continuous.And the fermentation was halfway.But continuous fermentation was convenient for industrialization producing.Although the residual glucose content in continuous fermentation mode was high through 0.06 h-1 sugar concentration,the last translation rate of ethanol was high.