Ferula ferulaeoides is a highly valuable medicinal plant native to Mongolia. In vitro seed germination effi ciency of F. ferulaeoides was low, and it required cold stratifi cation for longer than 21 days and exogenous application of the hormone gibberellic acid (GA3) for germination. Cotyledons, hypocotyls and roots of two week old seedlings were cultured on Murashige and Skoog (MS) media supplemented with diff erent auxins including 2,4-dichlorophenoxy acetic acid (2,4-D), napthalene acetic acid (NAA) and indole-acetic acid (IAA) and cytokinin 6-Benzylaminopurine (BAP). This experiment showed that combination of BAP (0.5 mg/L) and auxins IAA and 2, 4 D was the most effi cient media for callus initiation from in vitro germinated seedling explants. Callus was sub-cultured with 4 weeks interval on the same media as callus initiation for a long term maintenance.