The capacity of LiDAR and Unmanned Aerial Vehicles (UAVs) to provide plant height estimates as a high-throughput plant phenotyping trait was explored. An experiment over wheat genotypes conducted under well watered and water stress modalities was conducted. Frequent LiDAR measurements were performed along the growth cycle using a phénomobile unmanned ground vehicle. UAV equipped with a high resolution RGB camera was flying the experiment several times to retrieve the digital surface model from structure from motion techniques. Both techniques provide a 3D dense point cloud from which the plant height can be estimated. Plant height first defined as the z-value for which 99.5% of the points of the dense cloud are below. This provides good consistency with manual measurements of plant height (RMSE = 3.5 cm) while minimizing the variability along each microplot. Results show that LiDAR and structure from motion plant height values are always consistent. However, a slight under-estimation is observed for structure from motion techniques, in relation with the coarser spatial resolution of UAV imagery and the limited penetration capacity of structure from motion as compared to LiDAR. Very high heritability values (H2> 0.90) were found for both techniques when lodging was not present. The dynamics of plant height shows that it carries pertinent information regarding the period and magnitude of the plant stress. Further, the date when the maximum plant height is reached was found to be very heritable (H2> 0.88) and a good proxy of the flowering stage. Finally, the capacity of plant height as a proxy for total above ground biomass and yield is discussed.
Accurate estimation of leaf chlorophyll content (Cab) from remote sensing is of tremendous significance to monitor the physiological status of vegetation or to estimate primary production. Many vegetation indices (VIs) have been developed to retrieve Cab at the canopy level from meter-to decameter-scale reflectance observations. However, most of these VIs may be affected by the possible confounding influence of canopy structure. The objective of this study is to develop methods for Cab estimation using millimeter to centimeter spatial resolution reflectance imagery acquired at the field level. Hyperspectral images were acquired over sugar beet canopies from a ground-based platform in the 400-1000 nm range, concurrently to Cab, green fraction (GF), green area index (GAI) ground measurements. The original image spatial resolution was successively degraded from 1 mm to 35 cm, resulting in eleven sets of hyperspectral images. Vegetation and soil pixels were discriminated, and for each spatial resolution, measured Cab values were related to various VIs computed over four sets of reflectance spectra extracted from the images (soil and vegetation pixels, only vegetation pixels, 50% darkest and brightest vegetation pixels). The selected VIs included some classical VIs from the literature as well as optimal combinations of spectral bands, including simple ratio (), modified
We propose a population approach to model the beginning of the French COVID-19 epidemic at the regional level. We rely on an extended Susceptible-Exposed-Infectious-Recovered (SEIR) mechanistic model, a simplified representation of the average epidemic process. Combining several French public datasets on the early dynamics of the epidemic, we estimate region-specific key parameters conditionally on this mechanistic model through Stochastic Approximation Expectation Maximization (SAEM) optimization using Monolix software. We thus estimate basic reproductive numbers by region before isolation (between 2.4 and 3.1), the percentage of infected people over time (between 2.0 and 5.9% as of May 11 th , 2020) and the impact of nationwide lockdown on the infection rate (decreasing the transmission rate by 72% toward a R e ranging from 0.7 to 0.9). We conclude that a lifting of the lockdown should be accompanied by further interventions to avoid an epidemic rebound.
The diurnal dynamics of leaf-rolling in maize genotypes as measured by visual scoring are strongly correlated with corresponding measurements made using digital hemispherical photography, leading to the potential for development of high-throughput techniques for phenotyping in the field.
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