Tanhua et al. Ocean FAIR Data Services formats and made available through Web services is necessary. In particular, automation of data workflows will be critical to reduce friction throughout the data value chain. Adhering to the FAIR principles with free, timely, and unrestricted access to ocean observation data is beneficial for the originators, has obvious benefits for users, and is an essential foundation for the development of new services made possible with big data technologies.
Ocean
metaproteomics is an emerging field enabling discoveries
about marine microbial communities and their impact on global biogeochemical
processes. Recent ocean metaproteomic studies have provided insight
into microbial nutrient transport, colimitation of carbon fixation,
the metabolism of microbial biofilms, and dynamics of carbon flux
in marine ecosystems. Future methodological developments could provide
new capabilities such as characterizing long-term ecosystem changes,
biogeochemical reaction rates, and in situ stoichiometries. Yet challenges
remain for ocean metaproteomics due to the great biological diversity
that produces highly complex mass spectra, as well as the difficulty
in obtaining and working with environmental samples. This review summarizes
the progress and challenges facing ocean metaproteomic scientists
and proposes best practices for data sharing of ocean metaproteomic
data sets, including the data types and metadata needed to enable
intercomparisons of protein distributions and annotations that could
foster global ocean metaproteomic capabilities.
Over a century ago, the scientific research community first acknowledged the importance and value data hold beyond their original, intended use (Cajal, 1999). Geoscience data hold particular value, representing snapshots of Earth systems that are unique in both space and time, and as such are often irreplaceable. These data serve as records of the past and present, necessary to understand current, and predict future states, rates and processes of global systems. But the
The Enabling FAIR Data project has brought together a broad spectrum of Earth, space, and environmental science leaders to ensure that data are findable, accessible, interoperable, and reusable.
We present METATRYP
version 2 software that identifies shared peptides
across the predicted proteomes of organisms within environmental metaproteomics
studies to enable accurate taxonomic attribution of peptides during
protein inference. Improvements include ingestion of complex sequence
assembly data categories (metagenomic and metatranscriptomic assemblies,
single cell amplified genomes, and metagenome assembled genomes),
prediction of the least common ancestor (LCA) for a peptide shared
across multiple organisms, increased performance through updates to
the backend architecture, and development of a web portal (
).
Major expansion of the marine METATRYP database with predicted proteomes
from environmental sequencing confirms a low occurrence of shared
tryptic peptides among disparate marine microorganisms, implying tractability
for targeted metaproteomics. METATRYP was designed to facilitate ocean
metaproteomics and has been integrated into the Ocean Protein Portal
(
); however, it can be readily applied to other domains. We describe
the rapid deployment of a coronavirus-specific web portal (
) to aid in use of proteomics on coronavirus research during the
ongoing pandemic. A coronavirus-focused METATRYP database identified
potential SARS-CoV-2 peptide biomarkers and indicated very few shared
tryptic peptides between SARS-CoV-2 and other disparate taxa analyzed,
sharing <1% peptides with taxa outside of the betacoronavirus group,
establishing that taxonomic specificity is achievable using tryptic
peptide-based proteomic diagnostic approaches.
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