Lung cancer is a leading cause of cancer-related deaths, contributing to 18.4% of cancer deaths globally. Treatment of non-small cell lung carcinoma has seen rapid progression with targeted therapies tailored to specific genetic drivers. However, identifying genetic alterations can be difficult due to lack of tissue, inaccessible tumors and the risk of complications for the patient with serial tissue sampling. The liquid biopsy provides a minimally invasive method which can obtain circulating biomarkers shed from the tumor and could be a safer alternative to tissue biopsy. While tissue biopsy remains the gold standard, liquid biopsies could be very beneficial where serial sampling is required, such as monitoring disease progression or development of resistance mutations to current targeted therapies. Liquid biopsies also have a potential role in identifying patients at risk of relapse post treatment and as a component of future lung cancer screening protocols. Rapid developments have led to multiple platforms for isolating circulating tumor cells (CTCs) and detecting circulating tumor DNA (ctDNA); however, standardization is lacking, especially in lung carcinoma. Additionally, clonal hematopoiesis of uncertain clinical significance must be taken into consideration in genetic sequencing, as it introduces the potential for false positives. Various biomarkers have been investigated in liquid biopsies; however, in this review, we will concentrate on the current use of ctDNA and CTCs, focusing on the clinical relevance, current and possible future applications and limitations of each.

Noninvasive methods for precise characterization of the thermal properties of soft biological tissues such as the skin can yield vital details about physiological health status including at critical intervals during recovery following skin injury. Here, we introduce quantitative measurement and characterization methods that allow rapid, accurate determination of the thermal conductivity of soft materials using thin, skin-like resistive sensor platforms. Systematic evaluations of skin at eight different locations and of six different synthetic skin-mimicking materials across sensor sizes, measurement times, and surface geometries (planar, highly curvilinear) validate simple scaling laws for data interpretation and parameter extraction. As an example of the possibilities, changes in the thermal properties of skin (volar forearm) can be monitored during recovery from exposure to ultraviolet radiation (sunburn) and to stressors associated with localized heating and cooling. More generally, the results described here facilitate rapid, non-invasive thermal measurements on broad classes of biological and non-biological soft materials.

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This paper shows the recent work of the authors in the development of a time-domain FEM model for evaluation of the seal dynamics of a surface effect ship. The fluid solver developed for this purpose, uses a potential flow approach along with a stream-line integration of the free surface. The paper focuses on the free surface-structure algorithm that has been developed to allow the simulation of the complex and highly dynamic behavior of the seals in the interface between the air cushion, and the water. The developed fluid-structure interaction solver is based, on one side, on an implicit iteration algorithm, communicating pressure forces and displacements of the seals at memory level and, on the other side, on an innovative wetting and drying scheme able to predict the water action on the seals. The stability of the iterative scheme is improved by means of relaxation, and the convergence is accelerated using Aitken's method. Several validations against experimental results have been carried out to demonstrate the developed algorithm.

We present a new 2-noded beam element based on the refined zigzag theory and the classical EulerBernoulli beam theory for the static analysis of composite laminate and sandwich beams. The proposed element is able to take into account distortion effects due to shear elastic strains and can predict delamination. The element has four degrees of freedom per node. A 1 C cubic Hermite interpolation is used for the vertical deflection while a 0 C linear interpolation is employed for the other kinematics variables. The stiffness matrix and the load vector are calculated in explicit form using exact integration. The element is free from shear locking as confirmed with numerical tests on a wide range of the slenderness ratios. Numerical results show the ability of the EEBZ2 element to reproduce accurately the vertical deflection along the beam length and complex zigzag distributions of the axial displacement and the stresses across the thickness. Delamination effects are modeled by incorporating of an additional zigzag function corresponding to the kinematics of a zero thickness layer where delamination occurs. An example showing the capability of the new EEBZ2 element for accurately reproducing delamination effects is presented.