A collection of 12 microbial cultures, known to contain cytochrome P-450 monooxygenase or other degradative enzymes, was screened for their ability to degrade the Novartis Crop Protection Inc. developmental fungicide cyprodinil (CGA-219417; 4-cyclopropyl-6-methyl-N-phenyl-2-pyrimidinamine). Ten of the 12 cultures produced a monohydroxylated metabolite in yields ranging from 1.2 to 35.6%. The filamentous fungus, Beauveria bassiana ATCC 7159, produced a methoxylated glycoside of the monohydroxylated metabolite with a yield of 80%. Dihydroxylated metabolites and a molecular cleavage product, 4-cyclopropyl-6-methyl-2-pyrimidamine, were also detected in certain cultures. The overall results of the study indicated that cyprodinil was readily metabolized by a variety of microbial species. Metabolites generated by these cultures can potentially be used as analytical reference standards to support animal, plant, and soil metabolism studies.

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