No abstract

Rotaviruses are an important agent of diarrhea in piglets, primarily during nursing and post-weaning with severity. The aim of this study was to identify rotavirus G and P genotypes obtained from piglets reared at five piggeries in Belém, Pará State, Northern Brazil. Fecal specimens were collected from nursing and weaned piglets. A total of 172 samples were tested, of which 17 (9.9%) were positive for group A rotaviruses all of them from piglets kept at nursing and then were sequenced for the of VP7 and VP4 genotyping. The consistency of positive fecal samples were 53% (9/17) diarrheic, 23.5% (4/17) pasty and 23.5% (4/17) normal. The most common G genotype was G3 representing 53% (9/17), followed by G5 genotype (17%, 3/17). The P genotype recorded was P[23] corresponding to 23.5% (4/17). This study showed that rotaviruses circulated in swine herds in the metropolitan region of Belém, in Northern region. G3P[23] combination was recorded for the first time in Brazil.

This present study has aimed to provide a molecular characterization of rotavirus species A (RVA) and to determine the frequency of RVC and RVH in fecal specimens from nonvaccinated piglets, across 5 farms in the metropolitan mesoregion of Belém, within the Amazon region, Brazil. In the previous study, porcine RVA was found in 9.9% (17/172) during the years 2008-2009. Using RT-PCR, Sanger nucleotide sequencing, and phylogenetic analyses, the entire genomes of 17 Brazilian porcine RVA strains were analyzed. The results indicated that all strains shared the same constellation, with the exception of the VP3 and NSP3 genes, which were M2 and T7 genotypes, respectively. The VP1, VP2, NSP2, NSP4 and NSP5 coding segments clustered into the Wa-like constellation; genotype 1, and VP7, VP4, VP6 and NSP1 genes were typically of porcine origin. RVA genes infecting pigs presented a high degree of nucleotide similarity to strains from other hosts, including humans. Little is known about the zoonotic potential of rotaviruses in Brazil, but the results of this study show dynamic interactions between human and porcine rotavirus strains and constitute an increase in knowledge on the diversity of RVA strains, in animal reservoirs circulating in the Amazon region.

O objetivo do presente estudo foi determinar a prevalência de anticorpos em um rebanho de búfalos de rio da Ilha de Marajó para os vírus: Vírus da diarreia viral bovina, Herpesvírus bovino tipo 1 e Vírus da leucose enzoótica bovina. A técnica de soroneutralização foi  utilizada para detecção de anticorpos referentes aos Vírus da diarreia viral bovina e Herpesvírus bovino tipo 1 e o teste de imunodifusão em gel de ágar para detecção de anticorpos para o Vírus da leucose enzoótica bovina. A prevalência de anticorpos para o Herpesvírus bovino tipo 1 em bubalinos está muito próxima da prevalência observada em bovinos. O Vírus da diarreia viral bovina apresentou prevalência de anticorpos muito abaixo da média quando comparada com prevalência de anticorpos em bovinos e bubalinos, inclusive criados no Brasil. O Vírus da leucose enzoótica bovina mostrou prevalência de anticorpos muito baixa, porém, próxima dos parâmetros observados em búfalos no Brasil e no mundo, que divergem da prevalência observada em bovinos, mostrando provavelmente uma resistência natural dessa espécie ao vírus, fator esse que precisa ser mais bem elucidado.