No abstract

Introdução:As síndromes linfoproliferativas formam um grupo heterogêneo de neoplasias malignas com diferentes comportamentos clínicos, fatores patológicos e características epidemiológicas e podem ter seu diagnóstico geral com base na morfologia das células linfoides observadas no sangue periférico. Objetivo: Testar a factibilidade diagnóstica do método de imunofenotipagem por citometria de fluxo para síndromes linfoproliferativas a partir da definição de um painel mínimo de anticorpos.

Gastric cancer (GC) is a worldwide health problem, making it one of the most common types of cancer, in fifth place of all tumor types, and the third highest cause of cancer deaths in the world. There is a subgroup of GC that consists of tumors infected with the Epstein–Barr virus (EBV) and is characterized mainly by the overexpression of programmed cell death protein-ligand-1 (PD-L1). In the present study, we present histopathological and survival data of a thousand GC patients, associated with EBV status and PD-L1 expression. Of the thousand tumors analyzed, 190 were EBV-positive and the vast majority (86.8%) had a high relative expression of mRNA and PD-L1 protein (p < 0.0001) in relation to non-neoplastic control. On the other hand, in EBV-negative samples, the majority had a low PD-L1 expression of RNA and protein (p < 0.0001). In the Kaplan–Meier analysis, the probability of survival and increased overall survival of EBV-positive GC patients was impacted by the PD-L1 overexpression (p < 0.0001 and p = 0.004, respectively). However, the PD-L1 low expression was correlated with low overall survival in those patients. Patients with GC positive for EBV, presenting PD-L1 overexpression can benefit from immunotherapy treatments and performing the quantification of PD-L1 in gastric neoplasms should be adopted as routine.

Human papillomavirus (HPV) is the most common sexually transmitted infection and occurs worldwide, especially in underdeveloped countries. It’s also strongly associated with cervical cancer. Papanicolaou test is an important tool for early diagnosis of such cancer. Hence, our objective is to evaluate women's knowledge about Pap smear, as well as about HPV transmission and clinical manifestations. This literature integrative review used the following research platforms: PubMed/Medline, SciELO and Virtual Health Library (BVS). Among the articles analyzed there were a total of 7,441 women, which mean age was over 16 years. Twenty percent of those had only high school education. Regarding knowledge about HPV, only 22% of the participants knew about it and 27% reported knowing about the Papanicolaou exam. It is concluded that socioeconomic factors are extremely relevant because they influence the incidence of HPV in society, emphasizing that knowledge about the Pap test and its purpose directly influences women’s acceptance to perform it.