We present a review of deep generative models and their applications in peptide design.

In response to threats, animals display quiescent behaviors such as freezing and tonic immobility. How neuromodulatory states like stress affect these behaviors is not well understood. Here we describe a Caenorhabditis elegans quiescent behavior, post-response quiescence (PRQ), which is modulated by the C. elegans response to cellular stressors. Following an aversive mechanical or blue light stimulus, worms respond first by briefly moving, and then become more quiescent for a period lasting tens of seconds. PRQ occurs at low frequency in unstressed animals, but is more frequent in animals that have experienced cellular stress due to ultraviolet light exposure as well as in animals following overexpression of epidermal growth factor (EGF). PRQ requires the function of the carboxypeptidase EGL-21 and the calcium-activated protein for secretion (CAPS) UNC-31, suggesting it has a neuropeptidergic mechanism. Although PRQ requires the sleep-promoting neurons RIS and ALA, it is not accompanied by decreased arousability, and is not homeostatically regulated, suggesting that it is not a sleep state. PRQ represents a simple, tractable model for studying how neuromodulatory states alter behavioral responses to stimuli.

Supplementary Figure 1. UV exposure reduces lifespan. Lifespan of young adult WT animals after exposure to 1000 J/m 2 UV radiation (blue, n = 19) and same chip controls (black, n = 15). Dots represent days when animals were scored. Mean lifespans: UV-treated 5.2 ± 0.3 days, control 10.9 ± 0.9 days, p = 0.00008, Wilcoxon rank-sum test.