Raman spectroscopy is used for studying the composition of cell lipidome. However, the Raman approach suffers from limited distinguish power for different lipids. In recent years, isotopically labeled molecules are increasingly used to enhance the Raman resolution of similar compounds in biological samples. Here, we present Raman study of fully deuterated stearic acid (stearic‐d35), semi‐deuterated oleic acid (oleic d‐9), arachidonic acid with deuterated methine groups (arachidonic‐d8), and their hydrogenated analogs. Polarization properties and temperature dependences of CD stretching modes were investigated to reveal the capability of the isotopically shifted Raman peaks in molecular characterization. Sensitivity of Raman peaks to order–disorder transition corresponding was demonstrated for deuterated methine, methylene, and methyl groups. It was found that the isotopically shifted Raman peaks provide information about the orientation of deuterated Raman tags and their surroundings.

Biomimetic phospholipid mixtures are actively used as models of biological membranes and materials for drug delivery in biomedical tasks. One of the essential properties of membranes formed from complex phospholipid...