Background: Various efforts have been made to accelerate the wound healing process in white rats, one of which is using Chromolaena odorata. The leaves contain tannins, phenols, flavonoids, saponins, and steroids, which accelerate the wound healing process. The purpose of this study was to determine the effect of Chromolaena odorata leaf jelly extract on the wound healing process in the inflammatory and proliferative phases in white rats microscopically (Rattus norvegicus).  Methods: A total of 27 healthy adult male white rats aged 3-4 months old were given open wounds on the back measuring 2x2 cm. The rats were then divided into four groups, a group administered with jelly without Chromolaena odorata leaf jelly extract (P0) and groups administered with Chromolaena odorata leaf jelly extract with concentrations of 10% (P1) and 30% (P2), respectively. Chromolaena odorata leaf jelly extracts were given daily by rubbing them on the wound surfaces. Skin biopsies were collected and evaluated histopathologically on days 3, 7, and 14 post-treatment. The histological assessment of wound healing was based on epithelialization and collagen density using Nagaoka criteria. The data were analyzed by analysis of variance (ANOVA) and continued with the Duncan test. Results: The Duncan test results showed that the mean score of the degree of epithelialization and collagen density of wound tissue on days 7 and 14 was significantly higher in the P2 group compared to the P0 and P1 treatments, while the P1 group was not significantly different from P0.  Conclusions: In conclusion, the administration of Chromolaena odorata leaf jelly extract could accelerate the degree of epithelialization and collagen density in the wound healing process of the inflammatory and proliferative phases of white rats (Rattus novergicus).

Introduction Intraperitoneal adhesion is a pathological condition of adhesion between the omentum, intestine and abdominal wall. The purpose of this study was to determine the effect of adding dexamethasone into normal saline fluid to prevent intra peritoneal adhesion. Materials and methods This study used experimental post-test only control group design. There were four treatment groups using six white rats in each group: group I without administration of normal saline and dexamethasone, group II with administration of normal saline irrigation, group III by adding dexamethasone dose 0.2 mg/BW into 5 cc normal saline, and group IV by adding 0.5 mg/BW into 5 cc normal saline. Laparotomy was performed to all samples followed by excoriation and abrasion in cecum and terminal ileum using gauze. Intra peritoneal adhesion assessment was carried out on the 10th day post laparotomy. Macroscopic and microscopic assessments were performed to evaluate formation of collagen, fibrin and the spread of inflammatory cells of each group. Results From macroscopic observations, it was found that the average adhesion that occurred was 3.333; 2.333; 0.666; 0.333 by using statistical calculations with one-way ANOVA with P value of 0.000. Post-Hoc analysis showed that the administration of dexamethasone 0.5 mg/BW is proven to minimize the occurrence of adhesion between the 3 groups. Microscopic assessment of the formation of collagen, fibrin and the spread of inflammatory cells by one-way ANOVA produced P 0.000 in all three parameters, but the dose of dexamethasone administration between 0.2 mg/BW and 0.5 kg/BW did not prove significant in either group. Conclusion There is evidence that the addition of dexamethasone to normal saline as an irrigation liquid during laparotomy can reduce the occurrence of adhesion. However, the dose difference was not proven to be better in this study. Further studies are suggested to use more experimental animals.