Enterobacterial common antigen (ECA) is a conserved polysaccharide present on the surface of the outer membrane (OM) and in the periplasm of the many pathogenic bacteria belonging to Enterobacterales , including Klebsiella pneumoniae , Salmonella enterica , and Yersinia pestis . As the OM is a permeability barrier that excludes many antibiotics, synthesis pathways for OM molecules are promising targets for antimicrobial discovery.

Despite advancement of neural progenitor cell transplantation to spinal cord injury clinical trials, there remains a lack of understanding of how biological sex of transplanted cells influences outcomes after transplantation. To address this, we transplanted GFP-expressing sex-matched, sex-mismatched, or mixed donor cells into sites of spinal cord injury in adult male and female mice. Biological sex of the donor cells does not influence graft neuron density, glial differentiation, formation of the reactive glial cell border, or graft axon outgrowth. However, male grafts in female hosts feature extensive hypervascularization accompanied by increased vascular diameter and perivascular cell density. We show greater T-cell infiltration within male-to-female grafts than other graft types. Together, these findings indicate a biological sex-specific immune response of female mice to male donor cells. Our work suggests that biological sex should be considered in the design of future clinical trials for cell transplantation in human injury.

Urbanization has produced various social, environmental, and hydrological impacts, such as reduced biodiversity, increased urban temperatures, ecosystem degradation, air and water pollution, changes to hydrological processes, groundwater recharge alterations, increased prevalence of floods, vegetation removal, and potential increases in unstable soils. Finding solutions to mitigate the impacts of urbanization is of vital importance in the development and planning of cities, and particularly so for developing countries. One strategy gaining momentum is the use of green roofs and larger green areas (greater green cover under trees, with the purpose of increasing the permeable area) for runoff control. In this study, a simulation was carried out using the i-Tree Hydro software that involved the urban basin in the Fontibón area of Bogotá, Colombia, with the aim of observing the hydrological benefits of trees, green areas, and permeable zones. Five scenarios were proposed in which green roof coverage was implemented (20% and 50% increases in green areas in Scenarios 1 and 2), coverage under existing trees was enhanced (50% and 100% increase in Scenarios 4 and 5), and finally a complete removal of green zones in Fontibón was simulated (Scenario 3). The town is relatively susceptible to a reduction in its existing green areas, with an increase in total flow of more than 50% for one scenario considered. Thus, an increase in the permeable coverage under trees (50% and 100% increased coverage under existing trees) provided the best strategy for mitigating the impacts of urbanization by reducing the total, maximum, and average impervious flow by 3%, 4%, and 8%, respectively. Finally, an increase in permeable zones corresponding to plants was proposed via the implementation of green roofs. However, this strategy showed a response to the reduction in the lowest total flow at 1%. runoff processes [12], cause surface water quality and runoff degradation [13], and increase the peak during precipitation events [14], which can cause the overflow of storm drains or combined sewers [15]. Flows in urban basins may exceed those in less urbanized or non-urbanized basins by between 30% and 100% [16].Permeable zones and green structures in urban environments are fundamental components of the design and planning for sustainable cities. Various investigations have determined the role that these structures play in mitigating heat island effects, pollutant removal from air and runoff water, their influence on the carbon cycle, and greenhouse gas reduction [17,18]. Vegetation cover is necessary to avoid oversaturating the processes taking place within a water system (e.g., infiltration, runoff, evapotranspiration). Urban green areas are also used as a strategy to prevent flooding since they increase the permeable surface within basins [19,20], on the other hand, some research has shown that the green roof help to reduce the annual runoff volume by 40% and peak discharge by 15% [21]. The World Health Organization has therefore established as a...

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Background & Objective: Chronic excessive alcohol consumption causes white matter degeneration with myelin loss and impaired neuronal conductivity. Subsequent rarefaction of myelin accounts for the sustained deficits in cognition, learning, and memory. Correspondingly, chronic heavy or repeated binge alcohol exposures in humans and experimental models alter myelin lipid composition leading to build-up of ceramides which can be neurotoxic and broadly inhibitory to brain functions. Methods: This study examined the effects of chronic + binge alcohol exposures (8 weeks) and intervention with myriocin, a ceramide inhibitor, on neurobehavioral functions (Open Field, Novel Object Recognition, and Morris Water Maze tests) and frontal lobe white matter myelin lipid biochemical pathology in an adult Long-Evans rat model. Results: The ethanol-exposed group had significant deficits in executive functions with increased indices of anxiety and impairments in spatial learning acquisition. Myriocin partially remediated these effects of ethanol while not impacting behavior in the control group. Ethanol-fed rats had significantly smaller brains with broadly reduced expression of sulfatides and reduced expression of two of the three sphingomyelins detected in frontal white matter. Myriocin partially resolved these effects corresponding with improvements in neurobehavioral function. Conclusion: Therapeutic strategies that support cerebral white matter myelin expression of sulfatide and sphingomyelin may help remediate cognitive-behavioral dysfunction following chronic heavy alcohol consumption in humans.