PRSS23 (Serine Protease 23) is a vital serine protease that is related to various types of cancers. However, the correlation between PRSS23 and gastric cancer remains unclear. In our study, our objective is to identify the key biomarkers and signaling pathways by analyzing the RNA-seq data. The GSE204725 was created by the Illumina HiSeq 4000 (Homo sapiens). The gene enrichment study indicated the cAMP signaling pathway and Calcium signaling pathway are the main biological processes during the knockdown of PRSS23 in gastric cancer. Moreover, we identified several interactive genes including NCAM1, HIST1H2BD, VWF, HIST1H2BL, GATA1, RYR2, CHRD, PDE4B, HIST1H4B, and HIST2H4B. Our study may provide new knowledge on the treatment of gastric cancer.

The RHO guanine exchange factor ARHGEF2 has exchange activity toward RHOA, which is essential for the development of cancers such as liver cancer. However, the potential functions and mechanisms of ARHGEF2 in the progression of liver cancer are largely unknown. In this study, we identified the transcriptional landscapes of hepatocellular carcinoma cells treated with ARHGEF2 shRNAs. The gene enrichment assays such as KEGG and GO were used to further analyze the potential signaling pathways. Moreover, the PPI network and Reactome map were used to further identify the biological processes. The results showed that Alzheimer's disease disease (AD) and Cushing syndrome (CS) are the major signaling pathways involved in the ARHGEF2-shRNAs treated hepatocellular carcinoma cells. We identified the top ten interactive genes including ICAM1, APOE, LDLR, NAT10, HSPA1A, EDN1, CACNA1C, KCNMA1, SNAI1, and ELN. Our study may provide novel mechanisms for the treatment of liver cancer by inhibiting ARHGEF2.

ID2 plays an essential role in cell fate decisions. However, the mechanism of ID2 regulating the functions of hematopoietic stem cells is still unclear. Here, our objective is to figure out the key molecules and signaling pathways by analyzing the RNA-seq data. The GSE203076 was created by the Illumina HiSeq 2500 (Mus musculus). The KEGG and GO analyses indicated the Neuroactive ligand−receptor interaction and the Calcium signaling pathway are the top two signaling pathways in the ID2-affected stem cells. Moreover, we figured out several interactive genes including PPARG, FGF2, ATM, MMP9, SOX9, ATR, KIT, CCNB1, HIST1H2BJ, and NES. Our study may provide new knowledge on the study of ID2 on stem cells.