Patient survival, but not graft survival, was adversely affected by both pre-existing diabetes and by PTDM, particularly in those with an age less than 55 yr.

AR had a deleterious impact on graft survival, particularly if occurring after 90 d. AR episodes should therefore be divided into early and late phases. In view of the very poor graft survival associated with LAR, it is important to gain further insight into the main aetiological factors. Those such as suboptimal CyA blood levels and non-compliance with medication should be further investigated with the aim of developing more effective immunosuppressive regimens in order to reduce the incidence of LAR.

SUMMARY1. Extraneuronal uptake of noradrenaline (NA) was examined in the cat spleen first by perfusing with NA for 10 min, followed by a 2-min wash to clear the extracellular fluid, then measuring the amount retained, its subcellular distribution and the tissue components involved as revealed by the development of the characteristic fluorescence. Secondly, thin spleen slices were exposed to NA in vitro and the development of fluorescence in various structures, particularly arterial smooth muscle, measured.2. The cat spleen accumulated large quantities of NA and this, like the development of fluorescence, was concentration-dependent. After particle separation most of the retained amine appeared in the high-speed supernatant, with a lesser amount in the coarse granule fraction. There was little amine in either the mitochondrial or microsomal fraction. The microsomal fraction from unperfused spleens was rich in NA, presumably from storage granules from the adrenergic nerves. On an intermittent sucrose density gradient the NA-rich particles sedimented between 1-0 and 1-5 M sucrose, corresponding to the recently described dense granules from bovine splenic nerves.3. Fluorescence histochemistry revealed several tissues accumulating NA. At an NA concentration of 10-5 g/ml., arterial smooth muscle and endothelium showed intracellular fluorescence; at 10 4g/ml., collagen, the perimeter of the smooth muscle cells of the capsule-trabecula-vein system and the reticular cells forming the framework of the spleen developed fluorescence. In the reticular cells the fluorescence was intracellular. The fluorescence pattern on the perimeter of non-arterial smooth muscle corresponded to the pattern of basement membrane as shown by PAS staining.