Test excavations at prehistoric site 22MR539 in the North Central Hills uplands of Mississippi have provided evidence of the effects of bioturbation on artifacts within the soil. Artifact distribution and size data are compared to soil particle size and organic content to determine the soil developmental pathway. Progressive soil formation factors have formed a biomantle over a currently forming artifact stone line, while regressive factors have moved artifacts to a depth of more than 50 cm below surface over approximately 2000 years. The single-component, short-term nature of 22MR539 and many similar sites recorded in the area provide an ideal situation to further explore biological and mechanical factors in site formation in an upland setting. ᭧