A nematicidal toxin was purified fromPleurotus ostreatus NRRL 3526 grown on moistened, autoclaved wheat straw for 30 days at room temperature (21-33°C). The active compound, at a concentration of 300 ppm, immobilized 95% of test nematodes (Panagrellus redivivus) within 1 hr. Immobilized nematodes did not recover, even after being rinsed with deionized water. The toxin was identified astrans-2-decenedioic acid.
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