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Rafflesia zollingeriana Koorders is a protected, rare, and endemic plants to Meru Betiri National Park. However, its population was declined. So, the study was focused on the analysis of the strengths, weaknesses, opportunities, and threats of conservation of R. zollingeriana to recommend some conservation's strategy and programs. This study was conducted at Meru Betiri National Park and its surrounding 5 villages. Data were collected by interviews, survey, observations, and literature studies. Qualitative data was analyzed by reduction, presentation and drawing conclusions simultaneously, while the quantitative data was analyzed descriptively. Matrix for SWOT analysis was processed by SPSS 16. The result showed that there were 4 elements that identified as strength, 5 elements that identified as weakness, 5 elements that identified as opportunity and 4 elements that identified as threat. The internal factor's value was higher than the external factor. The result of the SWOT analysis indicated that it needs aggresive strategy through collaboration among stakeholders to optimize both strength and opportunites. While the recommended program are: socialization the importance of R. zollingeriana conservation and developing a collaborative R. zollingeriana ecotourism.

Sangiang Island had been established as a Nature Recreation Park (TWA) in Indonesia, that requires comprehensive data support for good management. The rise of marine tourism that increased in TWA Sangiang Island behoove to be a concern, especially in coral reef ecosystem. The study aimed to determined the condition of the coral cover and the biomass composition of coral reef fishes, then to identify parameters that affect coral health and the coral reefs health index in TWA Sangiang Island. The observation was carried out in area near to the estuary (Legon Waru), entry point for shipping (Tembuyung), and diving tourism (Legon Bajo). The highest of biomass of target fish was Legon Bajo (0.013 kg / ha) and the lowest was Tembuyung (0.002 kg / ha). Coral reefs health index values in each station varied from 1 (low) to 4 (high). Coral reefs health index in Legon Waru was strongly influenced by live coral cover, and also in Legon Bajo was influenced by live coral cover, rubble and biomass of target fishes, while in Tembuyung was much influenced by fleshy seaweed cover. The results indicated that the coral reef ecosystem on Sangiang Island wasn’t in good condition as a whole.

Abstract. Jakaria, Ulum MF, Lestari D, Akwila S, Sihite DEWT, Priyanto R, Muladno, Sumantri C. 2020. Investigating new SNPs of CAST, CAPN and SCD genes in 5’UTR of Bali cattle. Biodiversitas 21: 2971-2976. The present investigation aims to explore specific SNPs of Bali cattle, focusing mainly on CAST, CAPN, and SCD genes in 5'UTR. DNA of Bali cattle (25 individuals) from Breeding Centre of Bali cattle in Bali Province, Indonesia, were extracted according to the protocol of GenAID. Amplification of the CAST, CAPN, and SCD genes were designed using the Primer3 program based on reference sequences (access numbers AH014526.2, AH009246.3, and AY241932). Furthermore, the PCR product was sequenced through forward primer sequencing. The data sequence results were analyzed using the Bioedit, MEGA7, Popgen 3.2, and DnaSP6 V6 programs. The results showed that SNPs were detected at g.921C>T and g.922T>G in 5'UTR for the CAST gene between the reference of GenBank AH014526.2 and Bali cattle, but it was monomorphic within the population. The high polymorphism of SNPs was found in g.232G>T for the CAPN gene and g.134A>del for the SCD gene. However, the SNP g.246C>del SCD gene was detected as monomorphic in this region. Moreover, this result shows that low nucleotide diversities (Pi) for CAST, CAPN, and SCD genes in 5'UTR of Bali cattle were 0.00422, 0.00632, and 0.00449, respectively. This finding was important for developing marker-assisted selection (MAS) to improve the meat quality of Bali cattle.