Berginia ligulata is medicinally significant and endangered plant of the western Himalayan Region. This plant is used to treat many diseases such as urinary problems and heart problems. By using leaf explant, an efficient and rapid micropropagation protocol was developed. In in-vitro propagation, MS medium was supplemented with benzyl amino purine (BAP), 1-naphthalene acetic acid (NAA) and indol-3-acetic acid (IAA) to induce multiple shoots from leaf explants. The most positive response for the formation of calluses was observed on MS media supplemented with MS medium + BAP(1 mg/L) + NAA(1 mg/L) followed by MS medium + BAP (0.5 mg/L) + NAA (1 mg/L) after 3 weeks. Maximum number of shoots were obtained on MS medium + BAP (2 mg/L) +IAA (2 mg/L). Maximum % shoot response was obtained on MS medium + Kn (2.5 mg/L) concentration. The maximum number of roots was observed on MS medium without growth regulators. These well-rooted plants were established in pots and acclimatized in the lab and transferred to field conditions. This process is useful to produce endangered plants on large scale.

Rabdosia rugosa (Wall. ex Benth.) H. Hara. (Syn. Plectranthusrugosus Wall.) belongs to the Lamiaceae family and is used by local communities of the Kinnaur district to cure diabetes. The selection of the plant material is based upon data that aerial parts of the plant (leaves along with flowers) are used in folklore to manage the postprandial hike in blood sugar levels. Therefore, in the present investigation, antiradical and antidiabetic activities of the ethanolic extract of the plant were checked. Preliminary tests for phytochemicals, total phenols, and flavenoids were also determined. Our results manifested that R. rugosa (Rabdosia rugosa) extract exhibited superior antioxidant activity (p˂0.05) by DPPH and H2O2 assays with IC50 = 9.62 ± 2.70 μg/ml and IC50 = 28.22 ± 2.94 μg/ml. This plant also exhibited preeminent repression action against the enzyme, i.e., alpha-amylase and alpha-glucosidase (p˂0.05) with IC50 = 10.49 ± 6.17 μg/ml (Porcine α – amylase), IC50 = 1.87 ± 1.79 μg/ml (Yeast α - glucosidase), ( IC50 = 16.89 ± 0. 06 μg/ml(Intestinal rat α - glucosidase) Hence, we concluded that present plant possessed excellent antiradical and antidiabetic activity. The antidiabetic activity was estimated higher than the standard Acarbose. As per our information, this may be the first time to report on the extract of R. rugosa for its antioxidant and antidiabetic capacity. The current findings divulge that this plant can further be studied to verify its therapeutic activities.

Ethnobotanical exploration was undertaken to document the Wild edible plants (WEPs) utilised by the inhabitants of the Leh district. Traditionally exploited WEPs are an indispensable part of every household culinary. This place is known for its unique culture and cuisine which diversifies it from the rest of India. Pre-structured questionnaires, guided field visits, field examinations, and group discussions were conducted to gather ethnobotanical data. Detailed inquiries were made on the plant’s part used, time of collection and method of food preparation. In the present study, 40 wild consumable plants were documented, these belong to 18 families and spreads to 31 genera; out of which four are new records as wild food plants. They are Cotoneaster integerrimus, Dracocephalum heterophyllum, Astragalus frigidus, and Turritis glabra. Diverse use of wild leafy plants was observed and some of the most famous dishes are ‘Shangsho tsodma’ and ‘Kabra tsodma’ vegetables. The utilisation of wild plants helps enrich diet diversity and enhances the availability of green vegetables hence broadening food choices. Our study also reveals that the gathering of wild plants is confined to village people, shepherds, and farmers, and a majority of this knowledge exists in the memory of the elderly and these wild resources are under threat due to various anthropogenic activities. Therefore, the present finding highlights the value of these plants along with maintaining regional traditional knowledge and preserving the old ethnic traditional way of living and eating. Further, this information will provide baseline data to upcoming researchers dealing with nutrition and nutraceutical aspects. In addition, these wild plants are nutritionally rich and their consumption should be encouraged.