Valley pseudospin, the quantum degree of freedom characterizing the degenerate valleys in energy bands 1 , is a distinct feature of two-dimensional Dirac materials [1][2][3][4][5] . Similar to spin, the valley pseudospin is spanned by a time reversal pair of states, though the two valley pseudospin states transform to each other under spatial inversion. The breaking of inversion symmetry induces various valley-contrasted physical properties; for instance, valley-dependent topological transport is of both scientific and technological interests [2][3][4][5] . Bilayer graphene (BLG) is a unique system whose intrinsic inversion symmetry can be controllably broken by a perpendicular electric field, offering a rare possibility for continuously tunable valley-topological transport. Here, we used a perpendicular gate electric field to break the inversion symmetry in BLG, and a giant nonlocal response was observed as a result of the topological transport of the valley pseudospin. We further showed that the valley transport is fully tunable by external gates, and that the nonlocal signal persists up to room temperature and over long distances. These observations challenge contemporary understanding of topological transport in a gapped system, and the robust topological transport may lead to future valleytronic applications.In crystalline solids, a topological current can be induced by the Berry phase of the electronic wave function 6 . Examples include the quantum Hall current in a magnetic field, and the spin Hall current arising from spin-orbit coupling. Such topological transport is robust against impurities and defects in materials -a feature that is much sought after in potential electronic applications. In such applications, the ability to switch and to continuously tune the topological transport is crucial. The topological current is in principle dictated by the crystal symmetry, which is difficult to change in Page 3 of 16 bulk materials. Bilayer graphene, however, offer new opportunities in which inversion symmetry can be controllably broken by an external electric field in the perpendicular direction.The topological current controlled by the inversion symmetry breaking is associated with carriers' valley pseudospin, which characterises the two-fold degenerate band-edges located at the corners of the hexagonal Brillouin zone. The topological Hall current, odd under time-reversal but even under inversion, is strictly zero in pristine mono-and bi-layer graphene which respect both symmetries. When the inversion symmetry is broken, however, time-reversal symmetry requires the Hall currents to have opposite signs and equal magnitudes in the two valleys (i.e., a valley The nonlocal transport persists up to room temperature and over long distances (up to 10 m). Our results represent major progress in the quest for a robust, tunable valley pseudospin system among various alternatives [3][4][5]11,12 , and indicate the possibility of using the nonlocal topological transport in practical applications under ambient conditio...