screening of 25 SSR markers, revealed 23 clear and consistent amplification profiles in the entire walnut germplasm set. A total of 54 alleles were amplified by SSR primers and the number of alleles range from 2 to 3. The PIC value ranged from 0.36 to 0.68. The dendrogram classified all genotypes into two main clusters with various degrees of subclustering. Estimated genetic dissimilarity coefficient ranged from 0.36 to 0.85. Through model-based cluster analysis all genotypes were grouped into 5 genetically distinct subpopulations. The expected heterozygosity at a given locus was found to range from 0.520 to 0.5477. Similarly, population differentiation measurements (Fst) ranged from 0.2286 to 0.2909. These findings would be helpful for decision making in future walnut breeding studies, germplasm management activities to maximize genetic diversity in walnut germplasm and may also prove useful in future for conducting association mapping in walnut for different traits.
The experiment was carried out during 2008 to 2009 and 2009 to 2010 with the aim to study the effect of girdling and foliar application of zinc and boron on growth, quality and leaf nutrient status of olive cv. Frontoio. The experiment was laid out in a randomized block design with seventeen treatments. Maximum shoot extension growth (8.92 cm) was observed with the spray of 0.6% ZnSO 4 in combination with 0.6% boric acid. Maximum pulp:stone ratio of 2.57 and oil content of 25.62% was recorded with the foliar application of 0.6% ZnSO 4 in combination with 0.6% boric acid and 0.6% ZnSO 4 in combination with 0.4% boric acid, respectively. The highest leaf K (1.74%), Ca (1.71%) and Mg (0.15%) contents were recorded with spray of 0.5% ZnSO 4 in combination with 0.4% boric acid, 0.5% ZnSO 4 in combination with 0.5% boric acid and 0.6% ZnSO 4 in combination with 0.6% boric acid, respectively. From this study, it is concluded that foliar application of zinc sulphate in combination with boric acid resulted in better growth, physical characteristics and oil content of olive fruits.
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