The article presents a technique of taking a genetic material from a postmortem material of appendage and testes of sables, conducted research to determine the mobility of sperms, morphological assessment and survival of sperms outside the animal body. When introducing artificial insemination in the sable industry, the use of post-mortem genetic material obtained from male sables is a promising direction in the reproduction of sable as a particularly valuable commercial species, as well as for its introduction into the zoo culture in order to ensure the biodiversity of natural systems where these animals live. It can also be used in the development of programs for the reproduction and conservation of populations of other particularly valuable, rare, and endangered species of mammals.

The purpose of our research was to study the effect of transcranial electrical stimulation on the regeneration of damaged nerve fibers in laboratory animals. In the experiment, an operation was performed to cut the sciatic nerve at the level of the midthigh and to connect its ends with an epiperineural microsurgical suture. After the operation, one group (experimental) of animals was subjected to electrical exposure using electrodes inserted subcutaneously in the forehead and behind the auricles. The electric current was supplied in the mode of a combination of a direct current of 0.8±0.2 mA and a pulse current of 0.4±0.1 mA with a frequency of 70 Hz, a pulse duration of 3.0-3.5 ms. There were 4 sessions of exposure lasting 1 hour with an interval of three days for two weeks after the operation. The animals were monitored for 9 months. Transcranial electroanalgesia (electrostimulation) was found to activate regenerative morpho-functional processes in the nerve fibers of the damaged sciatic nerve in rats.

The article is devoted to the problem of purulo-necrotic lesions of the distal extremities, including necrobacteriosis, the study of the role of concomitant bacterial microflora in the occurrence of the disease, as well as the development of new, more effective products safe for animals and product quality. The therapeutic efficacy of the drug Ceftiofur-VS was studied as a result of its use in purulo-necrotic pathologies in the hoof area in cattle. Studies on the comparative therapeutic effectiveness of the drug Ceftiofur-VS in necrobacteriosis in cattle, complicated by associations of microorganisms, were conducted in 2019-2020 in the farms of the Irkutsk region. The objects of the study were 74 cows with purulo-necrotic hoof lesions, with a laboratory-confirmed diagnosis of necrobacteriosis, which were divided into two groups: experimental (50 heads) and control (24 heads). It was found that when using the drug Ceftiofur-VS, a positive therapeutic effect occurred in 46 cows and amounted to 92 %. In the animals of the experimental group, signs of healing were noted already on the 4th day after the start of treatment: the inflammatory reaction (edema and hyperemia) and the affected area significantly decreased, fully cleared from the purulo-necrotic substrate. On the 5th day, young granulations appeared, on the 7th-8th day, signs of purulent inflammation were significantly leveled, exudation from wounds stopped, and marginal epidermization was noted. The average area of the wound was on average about 40% of the original one. The recovery time of animals with necrobacteriosis complicated by associations of microorganisms was, on average, 11-12 days.

The objective of our study was to establish the possibility of safe and painless castration of males using only sedatives together with local anesthesia. Animals, before appropriate fixation, were intramuscularly injected with xylanit (active substance xylazine) at a minimum dose of 1 mg/kg of live weight and zoletil at a subanesthetic dose of 1 mg/kg of animal weight, the testes and scrotal skin were infiltrated with 2% lidocaine solution according to the generally accepted method in veterinary surgery. Before, during, and after surgery, nociception indicators were evaluated: blood pressure; heart rate and respiration rate; degree of pain relief depth (presence of pain and spontaneous movement). During the surgery, the heart rate decreased, on average, by 40-50 beats/minute from baseline values, the respiratory rate decreased, on average, by 10 breaths/minute, and there was a decrease in blood pressure. We found that pre-sedation of animals with xylanite and subanesthetic doses of zoletil followed by tissue infiltration with lidocaine allowed humanely performing orchidectomy, as well as minimizing the cost of general anesthesia and the duration of anesthesia aftereffect on the body.