The work carried out a morphometric study of the thymus of rabbits vaccinated with the associated vaccine against myxomatosis and viral hemorrhagic disease of rabbits (VHD) at a dose of 0.5 ml subcutaneously and treated with an insecticide preparation “Stronghold” (selamectin 6%) at a dose of 0.1 ml / kg at the withers … Chinchilla rabbits, live weight 2-2.5 kg, aged 6-8 months were used as experimental material. In total, 20 animals were examined. The thymus was examined by conventional histological methods. The nature of changes in the morphofunctional parameters of the thymus with the combined use of the investigated factors revealed a dependence on the time of application of the drug “Stronghold” in relation to the vaccine. Our studies on rabbit immunogenesis with simultaneous vaccination against myxomatosis and viral hemorrhagic disease of rabbits (VGBD) and treatment with the Stronghold insecticide with the active ingredient selamectin in the form of a 6% solution did not show significant morphofunctional changes in the thymus with increased stress load on a living organism.
The analysis of the incidence of livestock (cattle and pigs) of salmonellosis in the territory of the Irkutsk region during 2004-2016 was carried out. It was found that the incidence rate of cattle was 5.4±0.62 per 100 thousands livestock, pigs -4.0±0.04 0 /0000. The dynamics of reducing of the incidence of pigs was identified. Negative rates of increase in the incidence of animals were revealed. The epizootic index was 1.0. Salmonella of nine species (Salmonella dublin, S.enteritidis, S.choleraesuis, S.typhimurium, S.london, S.lindi, S.wernigerode and S.othmarschen) in cattle and five species (S.choleraesuis, S.enteritidis, S.typhimurium, S.rochdale and S.arizonae) in pigs were identified as the causative agent of the infection. S.dublin was dominated at the etiology of salmonellosis in cattle (75.1 %) (p≤0.05), S.choleraesuisin pigs (89.2±2.9 %) (p≤0.01). Salmonellosis in cattle was recorded in 11 districts of the Irkutsk region, in pigsin nine districts.
The article presents a technique of taking a genetic material from a postmortem material of appendage and testes of sables, conducted research to determine the mobility of sperms, morphological assessment and survival of sperms outside the animal body. When introducing artificial insemination in the sable industry, the use of post-mortem genetic material obtained from male sables is a promising direction in the reproduction of sable as a particularly valuable commercial species, as well as for its introduction into the zoo culture in order to ensure the biodiversity of natural systems where these animals live. It can also be used in the development of programs for the reproduction and conservation of populations of other particularly valuable, rare, and endangered species of mammals.
The purpose of our research was to study the effect of transcranial electrical stimulation on the regeneration of damaged nerve fibers in laboratory animals. In the experiment, an operation was performed to cut the sciatic nerve at the level of the midthigh and to connect its ends with an epiperineural microsurgical suture. After the operation, one group (experimental) of animals was subjected to electrical exposure using electrodes inserted subcutaneously in the forehead and behind the auricles. The electric current was supplied in the mode of a combination of a direct current of 0.8±0.2 mA and a pulse current of 0.4±0.1 mA with a frequency of 70 Hz, a pulse duration of 3.0-3.5 ms. There were 4 sessions of exposure lasting 1 hour with an interval of three days for two weeks after the operation. The animals were monitored for 9 months. Transcranial electroanalgesia (electrostimulation) was found to activate regenerative morpho-functional processes in the nerve fibers of the damaged sciatic nerve in rats.
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