Nicotine can cause atherosclerosis by activating nuclear factor-kB (NF-kB) pathway lead to induce proinflammatory cytokines release as C-reactive protein (CRP) main regulators. The increase of CRP can induce reactive oxygen species (ROS) and increase of malondialdehyde (MDA). Quercetin has been proven to have antiinflammatory and antioxidant effects. This study was conducted to evaluate effect of quercetin on serum CRP and MDA concentrations in rats induced by nicotine. This was a true experimental study with post test only control group design. Thirty six of male Wistar rats were divided into six groups. Group I as normal control received 1 mL/kg BW of NaCl 0.9% solution. Group II as negative control received 2 mg/kg BW of nicotine and Group III as positive control received 2 mg/kg BW of nicotine and atorvastatin at dose of 5 mg/kg BW. Group IV-VI as treatment groups received 2 mg/kg BW of nicotine and quercetin at dose of 25; 50 or 100 mg/kg BW, respectively. Nicotine was given subcutaneously whereas atorvastatin and quercetin were given orally once per day for 28 days, consecutively. Serum CRP and MDA concentrations were measured using Rat hs-CRP ELISA kit and TBARS assay kit, respectively. Data were analyzed using analysis of variance (ANOVA) continued using LSD post-hoc test. The results showed that quercetin reduced serum CRP and MDA concentrations in dose dependent manner. Serum CRP concentration on Group V (173.39 ± 34.85 ng/mL) and Group VI (114.15 ± 43.62 ng/mL) were significantly lower than that Group II (244.77 ± 37.95 ng/mL) (p<0.05). Furthermore, serum MDA concentration on Group IV (5.95 ± 0.11 mmol/mL), Group V (3.93 ± 0.09 mmol/mL) and Group VI (2.14 ± 0.09 mmol/mL) were significantly lower than that Group II (7.29 ± 0.06 mmol/mL) (p<0.05). In conclusion, quercetin reduces the nicotine-induced oxidative status in rats. ABSTRAKNikotin dapat menyebabkan atherosclerosis dengan mengaktifkan jalur nuclear factorkB (NF-kB) yang menginduksi pelepasan sitokin proinflamasi sebagai regulator utama C-reactive protein (CRP). Kenaikan CRP dapat menginduksi reactive oxygen species (ROS) dan meningkatkan malondialdehid (MDA). Quersetin terbukti memiliki efek antiinflamasi J Med Sci, Volume 48, No. 2, 2016 April: 81-88 82 dan antioksisdan. Penelitian ini dilakukan untuk mengkaji efek quersetin terhadap kadar serum CRP dan MDA tikus yang diinduksi nikotin. Penelitian ini merupakan penelitian eksprimental murni menggunakan rancangan post test only control. Tiga puluh enam ekor tikus dibagi menjadi enam kelompok. Kelompok I sebagai control normal mnerima larutan NaCl 0.9% 1 ml/kg BB. Kelompok II sebagai control negatif menerima nikotik 2 mg/kg BB. Kelompok III sebagai control positif menerima nikotin 2 mg/kg BB dan atorvastatin 5 mg/kg BB. Kelompok IV-VI sebagai kelompok perlakuan menerima nikotin 2 mg/kg BB dan quersetin berturut-turut 25; 50 dan 100 mg/kg BB. Nikotin diberikan secara subkutan sedangkan atorvastatin dan quersetin diberikan secara oral. Kadar CRP dan MDA serum ditetapkan menggunakan Rat ...