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Bacterial cellulose or biocellulose (BC) is cellulose produced from the activity of bacteria in the suitable growth media containing glucose as mainly carbon source. Due to its unique properties, BC is used for edible packaging. Many studies reported on anti bacterial activity on BC based-edible packaging against Escherichia coli and Staphylococcus aureus as as well as Listeria monocytogenes. This study reports the biodegradation of BC (sample A) based-film by using Aspergillus unguis and Paecilomyces marquandii. For comparison, BC film was added with CMC (sample B), with glycerol (sample C), with CMC and glycerol (sample D), respectively. Biodegradation was carried out using broth fermentation and solid substrate fermentation (SSF). BC films (1 cm x 1 cm) and fungi were fermented in 100 mL of sterile aquadest for 60 days with agitation (120 rpm at room temperature). For treatment in SSF, fungi were inoculated into BC films for 60 days at room temperature in petridish. Results showed that all compounds of films were still available based on FTIR results. The physical performance of films BC was in solid chewy (A), like chewy thread (B and C), and like powder (D) form. Results showed that both fungi biodegraded films through broth fermentation whereas no biodegradation activity on SSF. From SEM analysis, it showed that the film surfaces performed fine and smooth morphology.

Paecilomyces Marquand strain TP4 is a saprophytic fungus isolated from soil taken from Tangkuban Perahu Mountain at West Java Province, Indonesia. This research aims to identify the growth profile of P. Marquand strain TP4 and the antifungal activity against Fusarium sp. The antifungal compound is produced by the liquid fermentation method using tofu wastewater and molasses media. The fermentation broth is extracted by liquid-liquid extraction using n-hexane, ethyl acetate and methanol solvents. The result shows that the optimum activity achieved after 120 hours of fermentation. The fermentation broth has antifungal activity against Fusarium sp., where the inhibitory diameter was 20 mm. The total flavonoid content and total phenolic content of freeze-dry fermentation culture were 0.4839 mg/g and 1.171 mg/g, respectively. Ethyl acetate extract shows the highest antifungal activity with MIC 31.25 ppm and MFC 125 ppm. The FTIR spectrum shows the OH, aliphatic CH, CC, aromatic C=C, CH and C-O. Characterization of an antifungal compound based on phytochemical test and FTIR determine to be a phenolic group.