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The separation of rehydrated Saccharomyces cerevisiae and an alkyl polyglycoside surfactant by column flotation was studied as a function of wash water flow and cation concentration. Separation of cells and surfactant was measured under steady-state conditions. Surfactant recovery in the foam concentrate was in the range of 86-95%. Yeast cells were enriched in the foam concentration by a factor of up to 1 I , but the recovery only reached 55 %. The use of wash water was very effective for removing the cells from the foam, giving a good separation between the cells and the surfactant. Addition of chloride salts of Na, K, Ca, and Mg at concentrations in the range of 0.05-0.1 moUL increased both the enrichment and the recovery of yeast in the foam. The most effective salt levels for cell flotation, less than 0.1 moliL, were in the same range of concentration as the minimum electrophoretic mobility of the cells. Les separation de Sasccharomyces cerevisiae rkhydratk et d'un surfactant de polyglycoside alkyl par flottation en colonne a ttC 6tudiCe en fonction de 1'6coulement de I'eau de lavage et de la concentration cationique. La skparation des cellules et du surfactant a kt6 mesur6e en rkgime permanent. La rkcupkration du surfactant dans le concentre mousseux se situe entre 86 et 95 % . Les cellules de levure ont Ctt enrichies dans la concentration de mousse par un facteur allant jusqu'a 11, mais seule la rkcupiration atteint 5 5 % . L'utilisation de I'eau de lavage est trks efficace pour retirer les cellules de la mousse et donne une bonne separation entre les cellules et le surfactant. L'ajout de sels de chlorure de Na, k, Ca et Mg a des concentrations entre 0,05 et 0,1 mol/L augmente h la fois I'enrichissement et la rtcuptration de la levure dans la mousse. Les teneurs en sel les plus efficaces pour la flottation des cellules, infkrieures a 0,l mol/L, sont dans la m&me gamme de concentration que la mobilitk Clectrophorktique minimale des cellules.

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