Soil specimens obtained from several areas of Japan , which are closely located to or facing the Continental land of China, were examined for the distribution of Clostridium botulinum, especially pertaining to types A and B. A total of 266 specimens of Japan, when cultured, showed no type A or B toxicity, although 30 (11 .3%), 4 (1.5%), and 10 (3.8%) of the specimens showed C1, C2, and type E toxicities , respectively. On the contrary, types A and/or B toxicities were shown , by the same method, in 14 of 20 specimens of Shinkiang district, China. The highest number of C. botulinum cells found in one gram of soil specimen was 25 for type A and 10 for type B.Clostridium botulinum is divided into seven types on the basis of the serological specificity of the neurotoxins produced. The distribution of the types of C. botulinum throughout the world is indicated by reports of the types of C . botulinum associated with botulism in man and animals and also by studies of the occurrence of the various types of the organisms, or spores, in soil. In Asia , types A and B are found at high frequency in soil of China (7, 13), whereas, in soil of Japan adjacent to China, types A and B have hardly been found, and the great majority of the types found belong to type C or E (1,8,10).We have already demonstrated that a number of isolates from spoiled canned foods produced in Japan showed high DNA homology titers, ranging from 81 to 91%, to C. botulinum type A (5). Since then, we have attempted to isolate C . botulinum from soil of Japan, although none of these isolates exhibited type A or B toxicity .The present paper describes that, although C . botulinum types A and B were easily isolated from soil specimens of Shinkiang district , China, none of these types could be found in the areas of Japan located close to the Continental land of China . The distribution of other types of C. botulinum is also stated .