Abstract. This paper presents a design-space exploration of an application-specific instruction-set processor (ASIP) for the computation of various cryptographic pairings over Barreto-Naehrig curves (BN curves). Cryptographic pairings are based on elliptic curves over finite fields-in the case of BN curves a field Fp of large prime order p. Efficient arithmetic in these fields is crucial for fast computation of pairings. Moreover, computation of cryptographic pairings is much more complex than elliptic-curve cryptography (ECC) in general. Therefore, we facilitate programming of the proposed ASIP by providing a C compiler.In order to speed up Fp arithmetic, a RISC core is extended with additional scalable functional units. Because the resulting speedup can be limited by the memory throughput, utilization of multiple data-memory banks is proposed.The presented design needs 15.8 ms for the computation of the Optimal-Ate pairing over a 256-bit BN curve at 338 MHz implemented with a 130 nm standard cell library. The processor core consumes 97 kGates making it suitable for the use in embedded systems.

Cotton Verticillium wilt, caused by the notorious fungal phytopathogen Verticillium dahliae (V. dahliae), is a destructive soil-borne vascular disease and severely decreases cotton yield and quality worldwide. Transcriptional and post-transcriptional regulation of genes responsive to V. dahliae are crucial for V. dahliae tolerance in plants. However, the specific microRNAs (miRNAs) and the miRNA/target gene crosstalk involved in cotton resistance to Verticillium wilt remain largely limited. To investigate the roles of regulatory RNAs under V. dahliae induction in upland cotton, mRNA and small RNA libraries were constructed from mocked and infected roots of two upland cotton cultivars with the V. dahliae-sensitive cultivar “Jimian 11 (J11) and the V. dahliae-tolerant cultivar “Zhongzhimian 2 (Z2). A comparative transcriptome analysis revealed 8330 transcripts were differentially expressed under V. dahliae stress and associated with several specific biological processes. Moreover, small RNA sequencing identified a total of 383 miRNAs, including 330 unique conserved miRNAs and 53 novel miRNAs. Analysis of the regulatory network involved in the response to V. dahliae stress revealed 31 differentially expressed miRNA–mRNA pairs, and the up-regulation of GhmiR395 and down-regulation of GhmiR165 were possibly involved in the response to V. dahliae by regulating sulfur assimilation through the GhmiR395-APS1/3 module and the establishment of the vascular pattern and secondary cell wall formation through GhmiR165-REV module, respectively. The integrative analysis of mRNA and miRNA expression profiles from upland cotton lays the foundation for further investigation of regulatory mechanisms of resistance to Verticillium wilt in cotton and other crops.

The mixed lineage leukemia 3 (MLL3), a member of the mixed lineage leukemia (MLL) family, has been reported to be mutated in multiple cancer types. However, its function in esophageal squamous cell carcinoma (ESCC) remains poorly understood. Here, we found that the expression of MLL3 was downregulated in ESCC tissues. Moreover, over-expression of MLL3 in ESCC cells inhibited cell proliferation and migration, while the knockdown expression of MLL3 promoted the tumorigenicity of ESCC cells. Mechanistically, MLL3 regulated the expression of multiple growth-related and migration-related genes. Taken together, our study suggested that downregulation of MLL3 was very important in the progression of ESCC.

No abstract

The pressure–impulse diagram is commonly used to assess the damage level of structural components under explosion. Non-dimensional pressure–impulse diagrams referred to different failure modes was obtained using a new methodology in this article. Nine non-dimensional key parameters were first proposed on basis of the Euler beam theory. Considering the shear failure, an elastic–plastic method to calculate the dynamic response of reinforced concrete beam columns was then proposed for different failure modes. Three failure categories, for example, bending failure, shear failure, and combined shear and bending failure, were considered. The threshold between the three failure modes was determined using non-dimensional pressure–impulse curves. A systematic parametric study was conducted to investigate the effects of different non-dimensional parameters on the dynamic response and the failure modes of reinforced concrete beam column. Parametric study shows that the nine non-dimensional key parameters are sufficient to calculate the dynamic response of reinforced concrete beam columns. Moreover, present study shows that the tangent modulus of direct shear stress–slip relation has a great influence on the failure modes. Beam columns with a smaller tangent modulus are more likely to generate combined shear and bending failure mode.