Technology , 55, 174-181. DOI: 10.1016, 55, 174-181. DOI: 10. /j.postharvbio.2009. 9 10 You may download, copy and otherwise use the AAM for non-commercial purposes provided that your 11 license is limited by the following restrictions: 12 13 (1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND 14 license.
15(2) The integrity of the work and identification of the author, copyright owner, and publisher must be 16 preserved in any copy. (Janisiewicz and Korsten, 2002). Some filamentous fungi (Melgarejo et al., 1986; Hong et al., 1998), 87 yeasts (Spotts et al., 2002; Karabulut and Baykal, 2003; Fiori et al., 2008), and bacteria (Pusey and 88 Wilson, 1984; Smilanick et al., 1993; Bonaterra et al., 2003) Redhaven harvested at commercial maturity. They were disinfected in sodium hypochlorite (NaClO,
145To select potential biocontrol agents, the isolates were tested directly on wounded peaches 146 according to Lima et al. (1999) DNA as a template and universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4
165(5'-TCCTCCGCTTATTGATATGC-3'). The D1/D2 domains were amplified using the primers NL-166 1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and NL-4 (5'-GGTCCGTGTTTCAAGACGG-167 3') on the genomic DNA. PCRs were performed using a TGradient thermal cycler (Biometra, 168 Göttingen, Germany Results were reported as mg ascorbic acid/100 g sample. Acidity was measured by titration with 0.1 242 N NaOH to pH 8.0: 5 mL of pressed juice diluted with 5 mL of distilled water were evaluated.
243Titratable acidity was calculated as percent malic acid (Wright and Kader, 1997).
244Three replicates of 60 peaches were used for each treatment. The experiment was repeated twice. regions were deposited in GenBank (accession numbers and amplimer sizes are indicated in Table 283 2
Molecular and morphological identification
Effect of storage temperature on biocontrol efficacy
320To determine the effects of different storage temperatures on the biocontrol efficacy against brown 321 rot, the peaches inoculated with the pathogen and treated with antagonists were stored at 20°C for 7 322 days, at 8°C for 14 days, and at 4°C for 21 days. After storage at 20°C (Fig.1), the antagonists AP6, 323 AP47, and PL5 reduced the diameter of brown rot lesions, respectively, to 13.7 mm, 24.5 mm, and 324 24.6 mm, compared to the control (49.4 mm). When the peaches were stored at 8°C (Fig. 1-A), the 325 antagonists more effectively reduced the diameters of brown rots: AP6, AP47, and PL5 reduced the 326 severity of brown rot to 8.8 mm, 10.6 mm, and 13.1 mm, respectively, compared to 54.3 mm of the 327 control fruit. After 21 days of storage at 1°C (Fig. 1-A), AP6, AP47, and PL5 provided the best 328 efficacy, by reducing the lesion diameters from 47.4 mm (in the inoculated fruit) to 2.2 mm, 2.1 mm, 329 and 9.1 mm, respectively. were treated with the three antagonists at various concentrations ( Fig. 1-B). At the concentration of 335 1×10 8 cells/mL, the three strains provided the highest biocontrol: the d...
