Efficacy of the antagonist Aureobasidium pullulans PL5 against postharvest pathogens of peach, apple and plum and its modes of action

Zhang, Dianpeng; Spadaro, D.; Garibaldi, A.; Gullino, M. L.

doi:10.1016/j.biocontrol.2010.05.003

Cited by 103 publications

(64 citation statements)

References 41 publications

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“…Previous experiments have shown that A. pullulans inhibited P. expansum and M. laxa in low concentrations of apple and peach juices, respectively [47,50]. However when the concentrations of juices were higher, nutrient competition was no longer an issue and A. pullulans was unable to control the pathogens.…”

Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

“…However, it might be that any bioactive compounds against M. laxa were produced but were not stable after they were secreted at room temperature. Zhang et al [47] reported that A. pullulans PL5 completely inhibited spore germination of M. laxa tested in PDB, whereas dead cells or cell-free broth had no effect. Bioactivity of A. pullulans has been assigned to two mechanisms: (1) secretion of lytic enzymes and (2) competition for nutrients [48].…”

Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

“…Bioactivity of A. pullulans has been assigned to two mechanisms: (1) secretion of lytic enzymes and (2) competition for nutrients [48]. Enzymes produced by A. pullulans were intensively studied for their bioactivity [47,48] but these enzymes were enriched using optimum conditions to maximise the quantity of enzymes producedwhich it is not likely to happen in a natural situation. Vero et al [49] suggested that enzymes did not seem to play a major role in inhibiting pathogens or their spore germination because A. pullulans took at least 48 h to produce them in the log phase or even longer (7 to 10 days) if the culture was incubated at 5 °C.…”

Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

“…The effect of B91 and Y126 on spore germination of M. laxa was assessed using the method modified from Zhang et al [47]. B91 and Y126 were grown in NB and PDB, respectively, at 25 °C for 4 days at 200 rpm.…”

Section: Inhibition Of Spore Germinationmentioning

confidence: 99%

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Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit

Rungjindamai

Jeffries

2013

Agronomy

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Abstract:Monilinia laxa is the causal agent of brown rot disease on stone fruits, and also causes blossom wilt and twig canker. The common practice used to manage this disease is through fungicide treatments. However the demand to reduce fungicide inputs has been increasing and there is a growing number of reports of M. laxa strains that are resistant to fungicides. There is an urgent need to search for an alternative strategy to control the disease. This study focused on the isolation and characterisation of biological control agents (BCAs) using indigenous isolates isolated from cherries and plums collected within the UK. A total of 192 isolates were screened against two strains of M. laxa in a series of in vitro dual culture tests. From this in vitro screen, 12 isolates were selected for a subsequent in vivo screen on detached fruits, which then narrowed these isolates down to two potential BCAs. These two strains were identified as Bacillus amyloliquefaciens/subtilis (isolate B91) and Aureobasidium pullulans (isolate Y126). The capability of these two potential BCAs to grow and survive at a range of temperatures likely to be experienced under field and storage conditions was studied in order to gain knowledge for product formulation and field application. Bacillus sp. B91 was shown to be a mesophilic bacterium that could grow at 10-25 °C but suffered significant mortality at 0 and 5 °C, while A. pullulans Y126 was both mesophilic and psychrotolerant as it grew between 0-25 °C with the optimum at 20 °C. When all nutrients were removed, Y126 was able to survive for several weeks in all test temperatures (0-25 °C) but showed significant OPEN ACCESSAgronomy 2013, 3 686 mortality at 25 °C. The capability of B91 to survive at 20 and 25 °C was higher than at low temperatures (0-15 °C). In addition, the modes of action of the potential BCAs were studied. B91 was shown to produce soluble and volatile organic compounds that inhibited M. laxa, while A. pullulans Y126 did not produce inhibitory compounds, but appeared to inhibit the pathogen via competition for nutrients. This study shows that microbial antagonists against M. laxa can be found from indigenous sources and that they are capable of preventing brown rot disease in controlled conditions, thus demonstrating a potential to be developed into commercial products.

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Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

Section: Inhibition Of Spore Germinationmentioning

confidence: 99%

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Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit

Rungjindamai

Jeffries

2013

Agronomy

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“…Their activity does not generally depend on the production of toxic metabolites. Also, they do not produce allergic spores or mycotoxins, are easily cultivated on simple media and can be produced on a large scale as commercial preparations 14 . Several yeasts have been identified as postharvest biocontrol agents against different diseases on different crops [15][16][17][18][19][20][21][22][23] .…”

mentioning

confidence: 99%

Role of Antagonistic Yeast <i>Candida tropicalis</i> YZ27 on Postharvest Life and Quality of Litchi cv. Bombai

et al. 2018

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The efficacy of the antagonistic yeast (Candida tropicalis YZ27) as a biological control agent against postharvest natural decay and quality retention of litchi (cv. Bombai) was studied. Application of the yeast antagonist led to rapid colonization on the surface of the fruit and significantly reduced the natural decay incidence and severity following storage at ambient condition (28  2C, 78  1% RH) for six days compared to control fruit. Application of C. tropicalis YZ27 prevented weight loss of fresh fruit (8.2%), pericarp browning (8%) and reduction of anthocyanin pigments (11%) of the pericarp over control (12.4%, 96.0% and 38.5% respectively in untreated fruit). Quality attributes like total soluble solids, titratable acidity and ascorbic acid content of litchi fruit were 17.18%, 0.53% and 7.78 mg/100 g in C. tropicalis YZ27 applied fruits compared to 11.95%, 0.30% and 6.16 mg/100 g respectively. The results indicate that C. tropicalis YZ27 has great potential for development of commercial biocontrol formulations as an alternative of SO 2 fumigation to control postharvest decay and quality retention of litchi fruit.

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Dissecting the Mechanisms of Action of Biocontrol Agents to Control Postharvest Diseases of Fruit

Spadaro

2014

Biological Controls for Preventing Food Deterioration

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Efficacy of the antagonist Aureobasidium pullulans PL5 against postharvest pathogens of peach, apple and plum and its modes of action

Cited by 103 publications

References 41 publications

Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit

Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit

Role of Antagonistic Yeast <i>Candida tropicalis</i> YZ27 on Postharvest Life and Quality of Litchi cv. Bombai

Dissecting the Mechanisms of Action of Biocontrol Agents to Control Postharvest Diseases of Fruit

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