The aim of the present study was to identify a specific biological marker for the diagnosis of colorectal adenomas through the analysis of variations in serum protein profiling in colorectal adenoma patients. The study was conducted at the Renmin Hospital of Wuhan University (Wuhan, China) between September 2011 and May 2012. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was performed to compare the serum protein profiles of 50 patients with colorectal adenoma and 50 healthy individuals. The obtained protein profiles were analyzed using Biomarker Wizard software. Twenty protein peaks were identified to exhibit differences in average intensity between colorectal adenomas compared with normal controls, including peaks 8,565.84, 8,694.51 and 5,910.50 Da, in which the intensity between the patients and control individuals was significantly different. Two peaks, 8,565.84 and 8,694.51 Da, were observed to be highly expressed in the colorectal adenomas, however, expression was low in the control samples. By contrast, 5,910.50 Da expression was low in the colorectal adenomas and high in the controls. The results of the current study indicate that the three protein peaks may represent specific biomarkers for colorectal adenomas.

Epidemiological data show that there is a close relation of high fat diet and obesity to the raising incidence rate of colorectal tumor [1, 2] . Some studies suggest that high blood lipid is a higher colorectal adenoma and colorectal cancer risk factors [3] , but there are also some studies have reported high cholesterol and the incidence of colorectal adenoma was no correlation [4] . The relationship between dyslipidemia and colorectal adenoma is still no consensus view. A large number of studies have shown that lipid peroxidation is closely related to the occurrence of colorectal cancer. This study explored the plasma lipid levels and lipid peroxidation levels change and the relationship between colorectal adenoma, aimed to further clarify dyslipidemia and plasma lipid peroxidation levels and the relationship between colorectal adenoma. Materials and methods ObjectsA total of 150 individuals, non-related were studied. Cases diagnosed with colonoscopy of 100 patients with colorectal adenomas (all were confirmed with pathologic diagnosis), 52 cases were males, 48 females, average age was (56.6 ± 7.1) years old. The control case for the whole colonoscopy did not find lesions in 50 patients, 26 cases were males and 24 females, average age with (54.1 ± 6.9) years old. The groups were matched by age and gender. The cases that had a history of colon operation, colon malignant tumor, diabetes, hyperlipoidemia and the recent use of drugs that affect blood lipids, non-steroidal anti-inflammatory drugs and contraceptives were rejected. The histological categories of colorectal adenomas were tubular adenoma, tubulovillous adenoma that also was named as mixed adenoma and villous adenoma. All objects were Chinese people. The anus to the splenic flexure of the colon was defined as the distal colon, cecum to the splenic flexure of the colon was defined as the proximal colon. Fifty-one cases were distal colorectal adenomas, 44 cases were proximal colorectal adenomas and 5 cases were distal and proximal colorectal adenomas. The cases having Abstract Objective: The aim of this study was to investigate lipid disorders and lipid peroxidation associated with the malignant transformation of colorectal adenoma. Methods: Analyses were based on data from 100 subjects with histologically confirmed adenomas (cases) and 50 adenoma-free control subjects, all of whom had colonoscopy. The subjects were divided into two groups: those with no adenoma and those with adenoma. According to subsite of adenomas the subjects with adenoma were divided into group of distal adenoma and group of proximal adenoma. According to histology of adenomas the subjects with adenoma were divided into group of villiform adenoma and group of tubular + tubulo-villous adenoma. Among the groups, the serum levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and lipid peroxidation product malondialdehyde (MDA) were compared in all the patients. Results: Plasma total cholesterol...

Purpose: To investigate the beneficial effect of alpha-lipoic acid (ALA) during pregnancy and lactation on susceptibility to ovalbumin (OVA)-induced neonatal asthma, and the mechanism of involved.Methods: Pregnant BALB/c mice were administered ALA (1 % mixed with mouse chow) or standard mouse chow from 6th day of gestation to 21st day of lactation (postnatal). The offspring (neonatal pups) from the OVA and ALA+OVA groups were sensitized on 1st, 7th and 14th postnatal days (PNDs) via intraperitoneal (i.p.) injection of OVA (0.5 μg). Control mice pups were not exposed to OVA. On PND 21, all pubs were again exposed to 1 % OVA aerosol using a nebulizer.Results: Neonatal mice exposed to ALA showed a significant decline (p < 0.05) in the number of inflammatory cells (eosinophils), levels of inflammatory markers (IL-4, IL-13, IL-5 and TNF-α) as well as OVA-specific IgE and total IgE, when compared to neonatal mice from pregnant mice that did not receive ALA (control). Moreover, the antioxidant profiles of ALA-treated mice offspring were significantly improved (p < 0.05). Marked downregulation (p < 0.05) of the protein expressions of NF-κB p-p65 subunit and TNF-α were observed in ALA-treated mice pups.Conclusion: ALA exposure during pregnancy (maternal exposure) markedly decreases OVA-induced asthmatic airway inflammatory response in pups. Thus, ALA might be beneficial for use along with standard anti-asthmatic drugs in the management of pediatric asthmatic patients

Purpose: To investigate the beneficial effect of alpha-lipoic acid (ALA) during pregnancy and lactation on susceptibility to ovalbumin (OVA)-induced neonatal asthma, and the mechanism of involved.Methods: Pregnant BALB/c mice were administered ALA (1 % mixed with mouse chow) or standard mouse chow from 6th day of gestation to 21st day of lactation (postnatal). The offspring (neonatal pups) from the OVA and ALA+OVA groups were sensitized on 1st, 7th and 14th postnatal days (PNDs) via intraperitoneal (i.p.) injection of OVA (0.5 μg). Control mice pups were not exposed to OVA. On PND 21, all pubs were again exposed to 1 % OVA aerosol using a nebulizer.Results: Neonatal mice exposed to ALA showed a significant decline (p < 0.05) in the number of inflammatory cells (eosinophils), levels of inflammatory markers (IL-4, IL-13, IL-5 and TNF-α) as well as OVA-specific IgE and total IgE, when compared to neonatal mice from pregnant mice that did not receive ALA (control). Moreover, the antioxidant profiles of ALA-treated mice offspring were significantly improved (p < 0.05). Marked downregulation (p < 0.05) of the protein expressions of NF-κB p-p65 subunit and TNF-α were observed in ALA-treated mice pups.Conclusion: ALA exposure during pregnancy (maternal exposure) markedly decreases OVA-induced asthmatic airway inflammatory response in pups. Thus, ALA might be beneficial for use along with standard anti-asthmatic drugs in the management of pediatric asthmatic patients