The Cisayong area of ​​Tasikmalaya Regency is an area that is passed by the Citanduy River. Citanduy River has received a variety of inputs, that affect the river water quality and cause pollution. Macrozoobenthos can be used as an indicator of water pollution. This research aims to determine the spatial distribution of the macrozoobenthic community as a bioindicator of organic matter pollution in the Citanduy River, Cisayong Tasikmalaya Region, West Java. This research was conducted on January 2 - March 3, 2020. The research method used a field survey method. The data collection technique used purposive sampling by determining 4 stations based on environmental factors and land use around the river flow. The results of the macrozoobenthic spatial distribution along the Citanduy River were different at each observation location. At station 1 several intolerant species Heptagenia sp., Leptophelbia sp., and Hydropsyche sp. Were found, station 2 found several facultative species Melanoides tuberculata, Lymnea sp., Tarebia granifera, Sulcosfira testudinaria, Filopaludina javanica, and Pomacea canalicuta, station 3 found several tolerant species Chironomus sp., and Tubifex sp., station 4 were found several species of facultative and tolerant macrozoobenthos Melanoides tuberculata, Lymnea sp., Tarebia granifera, Sulcosfira testudinaria, Filopaludina javanica, Pomacea canalicuta, and Tubifex sp. The abundance of macrozoobenthos in the Citanduy River ranges from 1460-2540 ind/m2. Diversity index ranges from 1.53 to 1.79. The uniformity index ranges from 0.45 to 0.53. Species deficits at each station are different. The Morisita index ranges from 0.48 to 1.12 representing uniform categories and groups.