The marine microalgae Tetraselmis striata was cultivated in drilling waters with different salinities. Growth substrate optimization was performed while the effects of different pH, temperature, photoperiod and CO2 flow rate on biomass productivity and its composition were studied. Results showed that the strain grew better in 2.8% drilling waters employing the fertilizer Nutri-Leaf together with ΝaHCO3. A pH value of 8 resulted in high biomass productivity (79.8 mg L−1 d−1) and biomass composition (proteins 51.2% d.w., carbohydrates 14.6% d.w., lipids 27.8% d.w. and total chlorophylls 5.1% d.w.). The optimum cultivation temperature was found to be 25 ± 1 °C which further enhanced biomass productivity (93.7 mg L−1 d−1) and composition (proteins 38.7% d.w., carbohydrates 20.4% d.w., lipids 30.2% d.w., total chlorophylls 5.1% d.w.). Photoperiod experiments showed that continuous illumination was essential for biomass production. A 10 mL min−1 flow rate of CO2 lead to biomass productivity of 87.5 mg L−1 d−1 and high intracellular content (proteins 44.6% d.w., carbohydrates 10.3% d.w., lipids 27.3% d.w., total chlorophylls 5.2% d.w.). Applying the optimum growth conditions, the produced biomass presented high protein content with adequate amino acids and high percentages of eicosapentaenoic acid (EPA), indicating its suitability for incorporation into conventional fish feeds. In addition, this study analyzed how functional parameters may influence the uptake of nutrients by Tetraselmis.
In this study, orange pulp (OP) and/or organic Se were fed to broilers in order to investigate their effects on the performance, behavior, breast meat quality, and oxidative stability. A total of 240 chicks were allocated to four groups: a control group; an OP group, fed with OP at 50 g/kg of diet; a Se group, fed with organic Se at 0.15 ppm; and an OP + Se group, fed with OP and organic Se at 50 g/kg and 0.15 ppm, respectively. The selenium and OP + Se groups showed improved meat oxidative stability during frozen storage from 90 to 210 days (p < 0.05), whereas the performance and meat quality were unaffected by the dietary treatments (p > 0.05), apart from a reduction in the meat pH and the dressing percentage in the OP-supplemented groups (p < 0.05). A synergistic action between OP and Se was observed for the meat oxidative stability. The polyunsaturated fatty acid (FA) and α-linolenic acid (ALA) contents in the breast meat lipid fractions were increased in the OP groups (p < 0.05). Dietary intervention did not affect the feeding or drinking behaviors of the broilers (p > 0.05). The dietary supplementation of broiler chickens with the citrus industry byproduct orange pulp at 50 g/kg, along with organic Se at 0.15 ppm, beneficially improves the meat oxidative stability and the meat nutritional value, with no negative side effects on the performance or the meat quality.
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