Until now, Anopheles sp is a vector of Plasmodium sp as a parasite that causes malaria. Global climate change makes the development of the Anopheles mosquito unpredictable. Therefore, the use of plants as biological larvicides is an alternative to control the Anopheles population. A study aimed at obtaining the phytochemical content of the crude extract of Tagetes erecta L. and the toxicity of the extract against larvae of Anopheles sp. Tagetes erecta flowers are obtained from the city of Tomohon, North Sulawesi which is known as the city of flowers. Mosquito larvae were obtained by rearing method in the biology laboratory of FMIPA, Manado State University. This study applies a laboratory experimental method. Lethal concentration 50 was determined by probit analysis using SPSS IBM 20. The study consisted of the extraction of Tegetes erecta flowers using the maceration method, analysis of phytochemical content and toxicity tests of extracts on larvae of Anopheles sp. The results showed that the dominant phytochemical groups were alkaloids, flavonoids, saponins and tannins. The best LC 50 was obtained in ethanol extract, namely 43.073 mg / L.Keywords : Toxicity, Larvae, Anopheles, Tagetes erecta L.  Abstrak: Anopheles sp sampai saat ini merupakan vektor Plasmodium sp sebagai parasite yang menyebabkan penyakit malaria. Perubahan iklim global menyebabkan perkembangan nyamuk Anopheles sulit diprediksi. Oleh karena itu pemanfaatan tumbuhan sebagai larvasida hayati menjadi alternative penanggulangan populasi Anopheles. Telah dilakukan penelitian yang bertujuan mendapatkan kandungan golongan fitokimia ekstrak kasar Tagetes erecta L dan toksisitas ekstrak terhadap larva Anopheles sp. Bunga Tagetes erecta di peroleh dari Kota Tomohon Sulawesi Utara yang dikenal sebagai kota bunga. Larva nyamuk diperoleh dengan metode rearing di laboratorium Biologi FMIPA Universitas Negeri Manado. Penelitian ini menerapkan metode eksperimen laboratorium. Lethal concentration 50 ditentukan dengan analisis probit menggunakan SPSS IBM 20. Penelitian terdiri atas tahap ekstraksi bunga Tegetes erecta dengan metode maserasi, analisis kandungan golongan fitokimia dan uji toksisitas ekstrak pada larva Anopheles sp. Hasil penelitian menunjukkan bahwa golongan fitokimia yang dominan adalah alkaloid, flavonoid, saponin dan tannin. LC 50 terbaik diperoleh pada ekstrak etanol yaitu 43,073 mg/L.Kata kunci : Toksisitas, Larva, Anopheles, Tagetes erecta L.

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Cats are the most domesticated animals kept by humans in the world. Cat fleas are ectoparasites that have the potential to transmit disease caused by microbes in humans. Biochemical identification research and antibiotic resistance tests have been carried out against bacterial isolates from cat fleas. Cat fleas were isolated from cats in Manado City, North Sulawesi, Indonesia. Isolation of bacteria was conducted using the scratch method on nutrient agar media. The pure bacterial culture is then used for biochemical analysis and antibiotic resistance testing. Biochemical analysis and resistance tests were performed using the Vitec 2 Compact instrument with a standardized automatic analysis model. The results obtained three species of bacteria from cat fleas based on biochemical identification, namely S. equorum, C. freundii, and Pantoea spp. Antibiotic resistance test on S. equorum showed that of the 60 types of antibiotics used, 55 were sensitive and 7 were resistant. Furthermore, C. freundii, of the 18 types of drugs, 7 were sensitive and 11 were resistant. Meanwhile, in Pantoea spp., Sensitive and resistant drugs were not found. However, the results of this study prove that bacteria from cat fleas have the potential to infect humans with relatively high antibiotic resistance.

Background: One of the health fields that is currently developing in various countries in adopting information and communication technology (ICT) is e-health. Hermina Hospital has a hermina mobile application that is useful for facilitating outpatient services. Initial data collection conducted by researchers in March showed that 23% of outpatient visits from executive polyclinics were registered via this application. Therefore, the aim of this research is to analyze the usage behavior of online registration at Hermina Hospital.Methods: This research was a quantitative correlational descriptive study with a cross sectional approach. The data used in this study obtained from the questionnaire using the method of convenient sampling with involved the participation of 110 respondents. Independent variables of this study were performance expectancy, effort expectancy, social influence, fcailitating condition dan behavioral intention. Dependent variable of this study is use behavior. The instrument used in this study was a quesioner.Results: The results of the chi square test found that performance expectancy, usage behavior, and behavioral intention were significantly related to use behavior (p < 0.05). Based on the results of the logistic regression test, it was found that the dominant factor influencing the use behavior is behavioral intention with a value of Exp (B) or Odds Ratio (OR) 2.746 and level of significant <0.05.Conclusion: The conclusion of this study is that there is a significant relationship between behavioral intention, effort expectancy, and performance expectancy on the decision to adopt the Hermina mobile application.   Latar Belakang: Salah satu bidang kesehatan yang saat ini sudah berkembang di berbagai negara dalam mengadopsi teknologi informasi dan komunikasi (TIK) yaitu e-Kesehatan (e-health). Rumah Sakit Hermina memiliki aplikasi hermina mobile yang berguna untuk mempermudah pelayanan pasien rawat jalan. Penelitian ini bertujuan untuk menganalisis faktor-faktor yang mempengaruhi keputusan adopsi aplikasi pendaftaran online hermina mobile pada pasien rawat jalan poli eksekutif di Rumah Sakit Hermina Manado.Metode: Penelitian ini merupakan penelitian kuantitatif korelasional dengan pendekatan cross sectional. Penelitian ini dilaksanakan di Rumah Sakit Hermina Manado pada November - Desember 2021. Subyek dalam penelitian ini yaitu 110 pasien poli eksekutif. Variabel bebas dari penelitian ini yaitu performance expectancy, effort expectancy, social influence, facilitating condition dan behavioral intention. Variabel terikat dari penelitian ini adalah keputusan adopsi (use behavior). Instrumen yang digunakan yaitu kuesioner. Data hasil penelitian dianalisis secara univariat, bivariat, dan multivariat. Penyajian data dibuat dalam bentuk tabel dan narasi.Hasil: Hasil uji chi square ditemukan bahwa performance expectancy, effort expectancy, dan behavioral intention memiliki hubungan bermakna dengan keputusan adopsi (use behavior) (p<0,05). Berdasrkan hasil uji regresi logistik ditemukan faktor dominan berpengaruh terhadap keputusan adopsi pasien poli eksekutif adalah behavioral intention dengan nila Exp (B) atau Odds Ratio (OR) 2,746 dan nilai p<0,05.Kesimpulan: Terdapat hubungan yang signifikan antara faktor behavioral intention, effort expectancy, dan performance expectancy terhadap keputusan adopsi aplikasi hermina mobile pada pasien rawat jalan poli eksekutif di Rumah Sakit Hermina Manado.

Alcohol is one of the most widely abused substance in the world. Alcohol use is prevalent in the medical field as a preservative and antiseptic. Alcohol abuse can be bad for our health, and can lead to dependence. Alcoholism is the people who are addicted to alcohol. This study aims to obtain an overview of adolescent behavior in the consumption of alcoholic beverages in the village of Sapa environment II Tenga subdistrict, Minahasa southern disctrict. This study is a descriptive study using a survey method to get an overview of adolescent behavior towards alcohol consumption habits. Results and conclusions: Knowledge: 97.8% of adolescent know the bad effects of alcohol on health. Attitude: The adolescent agreed participation of the sociality community, parents, religious leaders, and security personnel in the prevention of the abuse of alcoholic beverages. Action: 71 of 93 adolescents admitted to consuming alcoholic beverages.Keywords: alcohol, alcoholism, youth (adolescent), behaviou.Abstrak: Alkohol merupakan salah satu zat yang paling banyak disalahgunakan diseluruh dunia. Penggunaan alkohol yang lazim pada bidang kedokteran adalah sebagai pengawet dan antiseptik. Penyalahgunaan alkohol dapat berakibat buruk bagi kesehatan, dan dapat menyebabkan ketergantungan. Alkoholisme adalah orang yang kecanduan pada alkohol. Penelitian ini bertujuan memperoleh gambaran umum dari perilaku remaja dalam mengkonsumsi minuman beralkohol di desa Sapa lingkungan II kecamatan Tenga kabupaten Minahasa Selatan. Penelitian ini merupakan penelitian deskriptif dengan menggunakan metode survey untuk mendapatkan gambaran perilaku remaja terhadap kebiasaan mengkonsumsi minuman beralkohol. Hasil dan Simpulan: Pengetahuan: 97,8% remaja mengetahui dampak buruk minuman beralkohol pada kesehatan. Sikap: Para remaja menyetujui peran serta masyarakat, orang tua, pemuka agama, dan aparat keamanan dalam pencegahan penyalahgunaan minuman beralkohol. Tindakan : 71 dari 93 remaja mengaku mengkonsumsi minuman beralkohol.Kata kunci: alkohol, alkoholisme, remaja, perilaku.