Zingiber cassumunar Roxb (Bengal ginger) is among the medicinal plants possessing immunomodulatory potentials. It contains curcumin and some volatile compounds that reportedly exhibit immunomodulatory activities. This study set out to examine and determine the effect of ethyl acetate fraction of Z. cassumunar rhizome extract on phagocytic activity of macrophages and lymphocyte proliferation in vitro. Z. cassumunar rhizomes were macerated with 96% ethanol solvent and then fractionated using ethyl acetate. Macrophages and lymphocytes were isolated from male Balb/C mice; the former was tested for its phagocytic activity using latex bead assay, while the latter was examined for its proliferation using the MTT assay. The extracts were prepared with different concentrations: 25, 50, and 100 μg/m, and the effects of these treatments on the test mice were compared to the negative control group. The results showed that the ethyl acetate fraction of Z. cassumunar rhizome extract elevated the phagocytic activity of macrophages, as evident from an increase in active phagocytes (SFA) and phagocytosis index. Extracts at 100 μg/mL produced the highest percentage of SFA and phagocytic index. Also, the ethyl acetate fraction of Z. cassumunar rhizome extract could increase lymphocyte proliferation significantly compared to the negative control (p<0.05). Overall, these activities prove that Z. cassumunar rhizomes have the potential as an immunomodulatory agent.