As an annual Muslim celebration, Eid al-Adha Feast is celebrated every year in Malang City. In 2019 feast, animals were sacrificed in Malang City consisting of 1202 cattle, 3572 goats and 302 sheep. Antemortem and postmortem examination were very important steps to ensure the quality of consumable animal product produced during the feast. The importance of these examination was to prevent zoonotic food-borne and transmissible disease from contaminated animal product to other livestocks nearby. Fascioliosis was listed into routine disease check due to its potential to reduce the quality of animal product, especially the liver. This study was held in 5 sub-districts in Malang City according to descriptive data collected by interview and structural questionnaire as well as visual observation. The result showed that fascioliosis happened in 26,03% of cattle, 2,93% of goats, and 1,32% of sheep being slaughtered. We also analized several risk factors influencing fascioliosis incidence rate such as body score condition and age. The fascioliosis tent to happen in medium and mature animal by age in respectively 31,62% of cattle, 54,28% of goats, and 75% of sheep. Thus, prevention and curative evaluation of fascioliosis should be continuously developed in order to provide excellent animal products.

Rats are reported to be the intermediate hosts and reservoirs of several zoonotic protozoal diseases. Trypanosoma lewisi is commonly reported in rats’ blood and is considered non-pathogenic protozoa in humans. However, some countries documented several cases in humans with T. lewisi infection. Another zoonotic protozoon that develops in rats and can be transmissible to humans is Toxoplasma gondii. We intended to present the morphology and morphometry of T. lewisi and T.gondii in wild rats collected around Malang City to explore the potential risk of transmission nearby. The rats were collected using single live traps followed by identification, sexing, age approximation, and body morphometry. All specimens were euthanized according to the standard procedure followed by blood and peritoneal fluid collection. The fluid smear preparation and Giemsa staining were performed to detect the presence of T. lewisi and T. gondii. Morphologic and morphometric analyses were conducted using ImageJ software. Among the collected 50 collected rats, 23 were identified as Rattus norvegicus (46%), 22 as Rattus rattus (44 %), and 5 as Mus musculus (1%). In the case of protozoans infection, ten individuals were infected with T. Lewisi (20%) from the blood smear check, whereas peritoneal fluid smear examination revealed an infection of T. gondii in a specimen (2%). Results of the study proved trypanosomiasis and toxoplasmosis in wild rats in Malang City. Though the clinical significance to human and public health impact is questionable, further research and surveillance of rodent-borne parasitic pathogens will provide more information for pre-emptive action.

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The purpose of this study was to compare the purity, concentration, and DNA band visualization of the isolated sample and PCR amplicon from three sample storage methods i.e. fresh frozen sample ( -20oC to -196oC), preserved in formalin, and paraffin wax. For this tissue samples were collected from the sample stored at frozen temperature -20oC, 10% NS formalin, and paraffin-embedded preparations, and Abs260/230 and Abs260/280 values and electrophoresis of 0.8% and 2% agarose gel visualization were analyzed. The results of the study showed a significant value of Abs260/280 for the isolated and amplified DNA purity. Among the tested three methods, frozen sample isolates and the PCR amplicon visualized a good DNA band. Meanwhile, the formalin-fixed and paraffinized tissue storage method showed a slightly lower quality DNA and no DNA band, respectively, while the PCR amplicon visualized a thin DNA band. In conclusion, all the tissue storage methods can be applied for DNA preservation and isolation, and the samples are successfully amplified on PCR examination.

Many animals species develop their gastrointestinal tube with special features to accommodate their natural diet to survive under adverse conditions including the nutrient absorption capability. Information related to the histologic description of various bird species' digestive organs based on their diet and its significance is yet limited. This study aimed to present a descriptive explanation of gastrointestinal organs of a changeable hawk-eagle (Nisaetus cirrhatu) and oriental honey buzzard (Pernis ptilorhynchus) as carnivorous, a southern cassowary (Casuarius casuarius) as an omnivorous, and a domestic chicken (Gallus gallus) as granivorous. In the current study, proventriculus (glandular stomach) and intestinal segments were microscopically examined and compared to understand the special histological features among avian species due to their important roles to digest the ingesta. The dissected specimens were preserved in 10% non-buffered formalin, then were processed through the common standard procedure of tissue processing and eventually stained with haematoxylin-eosin. Microscopic observation showed variation in shape and size of proventricular glandular architecture among raptors. The intestine muscular layer of the cassowary also showed distinct thickness among birds. These results of the study preliminary proved that variation in diet might affect the histologic features of avian gastrointestinal tracts.