This study aimed to develop and validate an instrument for nursing triage in occupational health services (ICEST). Exploratory factor-analysis techniques were used to remove redundant or non-endorsed items and identify the factor structure of the ICEST. Validation of the items, content, construct and reliability were also performed. The final ICEST consisted of 14 indicators and 71-graduated indexes. The ICEST showed to be reliable, consistent and satisfactory structure to be applied in occupational health services. keywords: validation studies; occupational health; nursing