Human hair dermal papilla (DP) cells are specialized mesenchymal cells that play a pivotal role in hair regeneration and hair cycle activation. The current study aimed to first develop three-dimensional (3D) DP spheroids (DPS) with or without a silk-gelatin (SG) microenvironment, which showed enhanced DP-specific gene expression, resulting in enhanced extracellular matrix (ECM) production compared with a monolayer culture. We tested the feasibility of using this DPS model for drug screening by using minoxidil, which is a standard drug for androgenic alopecia. Minoxidil-treated DPS showed enhanced expression of growth factors and ECM proteins. Further, an attempt has been made to establish an in vitro 3D organoid model consisting of DPS encapsulated by SG hydrogel and hair follicle (HF) keratinocytes and stem cells. This HF organoid model showed the importance of structural features, cell-cell interaction, and hypoxia akin to in vivo HF. The study helped to elucidate the molecular mechanisms to stimulate cell proliferation, cell viability, and elevated expression of HF markers as well as epithelial-mesenchymal crosstalks, demonstrating high relevance to human HF biology. This simple in vitro DP organoid model system has the potential to provide significant insights into the underlying mechanisms of HF morphogenesis, distinct molecular signals relevant to different stages of the hair cycle, and hence can be used for controlled evaluation of the efficacy of new drug molecules.

Decellularized corneas obtained from other species have gained intense popularity in the field of tissue engineering due to its role to serve as an alternative to the limited availability of high-quality donor tissues. However, the decellularized cornea is found to evoke an immune response inspite of the removal of the cellular contents and antigens due to the distortion of the collagen fibrils that exposes certain antigenic sites, which often lead to graft rejection. Therefore, in this study we tested the hypothesis that cross-linking the decellularized corneas with chondroitin sulfate may help in restoring the distorted conformationation changes of fibrous matrix and thus help in reducing the occurrence of graft rejection. Cross-linking of the decellularized cornea with oxidized chondroitin sulfate was validated by ATR-FTIR analysis. An in vitro immune response study involving healthy monocytes and differentiated macrophages with their surface marker analysis by pHrodo red, Lysotracker red, ER tracker, and CD63, LAMP-2 antibodies confirmed that the cross-linked decellularized matrices elicited the least immune response compared to the decellularized ones. We implanted three sets of corneal scaffolds obtained from goat, i.e., native, decellularized, and decellularized corneas conjugated with chondroitin sulfate into the rabbit stroma. Histology analysis, three months after implantation into the rabbit corneal stromal region, confirmed the restoration of the collagen fibril conformation and the migration of cells to the implanted constructs, affirming proper graft integration. Hence we conclude that the chondroitin sulfate cross-linked decellularized corneal matrix may serve as an efficient alternative to the allograft and human cadaveric corneas.

Considering a realistic situation, where electron-neutral collisions persist in plasma, analytical calculations are carried out for the Terahertz (THz) radiation generation by beating of two Super-Gaussian (SG) lasers of index p. The competency of these lasers over Gaussian lasers is discussed in detail with respect to the effects of collision and beam width on the THz field amplitude and efficiency of the mechanism. A critical transverse distance of the peak of the THz field is defined that shows a dependence on the index of SG lasers. Although electron-neutral collisions and larger beam width lead to the drastic reduction in the THz field when the SG lasers are used in the plasma, the efficiency of the mechanism remains much larger than the case of Gaussian lasers. Moreover, the higher index SG lasers produce stronger and focused THz radiation.

We carry out analytical calculations for the electric field amplitude and efficiency of the mechanism of terahertz (THz) radiation generated due to interaction of super-Gaussian lasers with collisional plasma. The contribution of magnetic field and the role of index of lasers for enhancing the field amplitude are uncovered under the effect of laser beam width and collision frequency between electrons and neutrals. Terahertz radiation with larger electric field is realized when lasers of higher index and lower beam widths interact with the plasma in the presence of an external static magnetic field. Under optimized parameters, THz field amplitude of about 10 7 kV m −1 and 6% efficiency of the mechanism can be realized using the lasers with field 5.0 × 10 10 V m −1 .