Continuous stochastic position perturbations are an attractive alternative to transient perturbations in muscle and reflex studies because they allow efficient characterization of system properties. However, the relevance of the results obtained from stochastic perturbations remains unclear because they may induce a state change in muscle properties. We addressed this concern by comparing the force and stiffness responses of isolated muscles of the decerebrate cat elicited by stochastic perturbations to those evoked by "step" stretches of similar amplitudes. Muscle stiffness during stochastic perturbations was found to be predominantly linear and elastic in nature for a given operating point, showing no evidence of instantaneous amplitude-dependent nonlinearities, even during large movements. In contrast, force responses evoked by step stretches were found to be mainly viscous in nature and nonlinear for larger stretches, with only a small maintained (elastic) component. Stiffness magnitude decreased with displacement amplitude for both stochastic and step perturbations. Our results are largely consistent with the crossbridge theory of muscle contraction, indicating that transient and continuous displacements evoke different, although functionally relevant, aspects of muscle behavior. These differences have several implications for the neural control of posture and movement, and for the design of perturbations appropriate for its study.

Spike discharges of skeletomotor neurons innervating triceps surae muscles elicited by white noise modulated transmembrane current stimulation and muscle stretch were studied in decerebrated cats. The white noise modulated current intensity ranged from 4.3 to 63.2 nA peak-to-peak, while muscle stretches ranged from 100 microns to 4.26 mm peak-to-peak. The neuronal responses were studied by averaging the muscle length records centered at the skeletomotor action potentials (peri-spike average, PSA) and by Wiener analysis. Skeletomotor spikes appeared after a sharp peak in PSA of the injected current, preceded by a longer-lasting smaller wavelet of either depolarizing or hyperpolarizing direction. The PSA amplitude was not related to the injected current amplitude nor showed any differences related to the motor unit type. The PSA amplitudes were virtually independent of the stretching amplitude sigma, after an initial increase with stretching amplitudes in the range of 15-40 microns (S.D.), or 100-270 microns peak-to-peak. Analyses of cross-spectra indicated a small or absent increase in gain with frequency in response to injected current, but about 20 dB/decade in the range 10-100 Hz in response to muscle stretch. The peaks of both Wiener kernels in response to current injection appear to decrease with the amplitude of injected current, but this decrease was not statistically significant. The narrow first-order kernels suggest that the transfer function between the current input and spike discharge is lowpass with a wide passband, i.e. there is very little change in dynamics. The values of the second-order kernels appear to be nonzero only along the main diagonal.(ABSTRACT TRUNCATED AT 250 WORDS)

Spike discharges of skeletomotor neurons innervating triceps surae muscles elicited by white noise modulated transmembrane current stimulation and muscle stretch were studied in decerebrated cats. The white noise modulated current intensity ranged from 4.3 to 63.2 nA peak-to-peak, while muscle stretches ranged from 100 microns to 4.26 mm peak-to-peak. The neuronal responses were studied by averaging the muscle length records centered at the skeletomotor action potentials (peri-spike average, PSA) and by Wiener analysis. Skeletomotor spikes appeared after a sharp peak in PSA of the injected current, preceded by a longer-lasting smaller wavelet of either depolarizing or hyperpolarizing direction. The PSA amplitude was not related to the injected current amplitude nor showed any differences related to the motor unit type. The PSA amplitudes were virtually independent of the stretching amplitude sigma, after an initial increase with stretching amplitudes in the range of 15-40 microns (S.D.), or 100-270 microns peak-to-peak. Analyses of cross-spectra indicated a small or absent increase in gain with frequency in response to injected current, but about 20 dB/decade in the range 10-100 Hz in response to muscle stretch. The peaks of both Wiener kernels in response to current injection appear to decrease with the amplitude of injected current, but this decrease was not statistically significant. The narrow first-order kernels suggest that the transfer function between the current input and spike discharge is lowpass with a wide passband, i.e. there is very little change in dynamics. The values of the second-order kernels appear to be nonzero only along the main diagonal.(ABSTRACT TRUNCATED AT 250 WORDS)