ABSTRACT In this study, a commercial product of natural silicates and yeast (NSY) and saponins from Yucca schidigera and Quillaja saponaria plants (SBP) were used to evaluate their nutritional effects on growth and health status of pacific white shrimp Litopenaeus vannamei. Four isonitrogenous and iso-lipidic experimental diets were formulated to contain 0%, 0.3% NSY, 0.2% SBP and 0.6% NSY. After 60 days, shrimp were sampled and standard haemato-immunological parameters were measured. The growth performances of shrimp were significantly affected by the dietary inclusion of NSY and SBP, whereas the inclusion of NSY and SBP provide a better growth compared to the control group. Additionally, the inclusion of NSY and SBP significantly enhance the total haemocytes count and lysozyme activity in shrimp compared to control. Therefore, NSY and SBP can be regarded as the functional ingredients in shrimp diet to improve the growth and non-specific immune function of shrimp.Keywords: Silicates and yeast, saponins, growth, health status, shrimp Litopenaeus vannamei ABSTRAK Pada kajian ini, produk komersial yang berbasiskan kepada komponen alamiah silikat dan ragi (NSY) dan saponins yang berasal dari tumbuhan Yucca schidigera dan Quillaja saponaria (SBP) digunakan untuk mengevaluasi pengaruhu nutrisi yang dimiliki terhadap pertumbuhan dan status kesehatan dari udang putih Litopenaeus vannamei. Empat pakan yang bersifat iso-nitrogen dan iso-lipid diformulasikan dengan perlakuan 0%, 0.3% NSY, 0.2% SBP and 0.6% NSY. Setelah 60 hari, udang kemudian diambil sampelnya dan protokol standard untuk uji hemato-immunologi dilakukan. Laju pertumbuhan udang secara signifikan dipengaruhi oleh keberadaan NSY dan SBP, dimana inklusi NSY dan SBP memberikan pertumbuhan yang lebih baik dibandingkan kontrol. Selain itu, penggunaan NSY dan SBP secara signifikan meningkatkan jumlah total hemosit dan aktivitas lisozim pada udang perlakuan dibandingkan kontrol. Oleh karena itu, NSY dan SBP dapat digolongkan sebagai bahan baku fungsional pada pakan udang untuk meningkatkan pertumbuhan dan fungsi imun non-spesifik pada udangKata kunci: Silikat dan ragi, saponins, laju pertumbuhan, status kesehatan, udang Litopenaeus vannamei

Objective: This study aimed to evaluate the combination of hairy eggplant (Solanum ferox) and bitter ginger (Zingiber zerumbet) on the production performance and hematological parameters of whiteleg shrimp (Litopenaeus vannamei). Materials and Methods: Four treatments were formulated in the test feed, where P1 (control + commercial vitamin C); P2 (120 ml/l combination of S. ferox and Z. zerumbet ); P3 (100 ml/l); and P4 (80 ml/l) 6,000 post-larvae shrimp with an average initial weight of 0.2 gm were randomly stocked in four groups, with three replications per treatment, and 500 were stocked in each pond with a total of 12 pounds. Results: Based on the results, there were significant differences in production performance (survival, absolute weight growth, specific growth rate, and feed conversion ratio). Biologically, the best performance was found in the P3 treatment (100 ml/l). In this treatment, the total number of hemocytes and the number of hyaline hemocytes were much higher, and this was not the case in the control treatment (P1), where the number of semi-granular and granular cells was significantly higher than the treatment group (p < 0.05) . Conclusions: This study confirmed that supplementation of 100 ml/l of S. ferox and Z. zerumbet could improve the production performance and hemato-immunological parameters of whiteleg shrimp, with functional potential to be developed in phytobiotic-based commercial diets for shrimp.