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Keywords:Bacilli, Food Safety B. cereus PCR Phenotypic

Light period significantly influences on the growth and development of plants. One of the major genes of photoperiod sensitivity is Ppd-D1, located on the chromosome 2D. The aim of the work was to determine the alleles and molecular structure of Ppd-D1 gene in samples from the collection of Ae. tauschii accessions, which have different flowering periods, and in 29 Ukrainian wheat varieties. Methods. We used methods of allele-specific PCR with primers to the Ppd-D1 gene, sequencing and Blast-analysis. Results. The collection of Ae. tauschii accessions and several varieties of winter and spring wheat was studied. The molecular structure of the allelic variants (414, 429 and 453 b. p.) of Ppd-D1b gene was determined in the collection of Aegilops. tauschii accessions. Conclusions. The Ppd-D1a allele was present in all studied varieties of winter wheat. 60 % of spring wheat is characterized by Ppd-D1b allele (size of amplification products 414 b. p.). Blast-analysis of the sequence data banks on the basis of the reference sequence of sample k-1322 from the collection of Ae. tauschii accessions has shown a high homology (80 to 100 %) between the nucleotide sequences of PRR genes, that characterize the A and D genomes of representatives of the genera Triticum and Aegilops

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Lactobacillus plantarum bacteria isolated from plant surfaces and dairy products are characterized by the clear antagonistic activity against some phytopathogens [4]. The ability of L. plantarum to inhibit bacteria and fungi is strain-specific [15].Antagonistic activity of lactobacilli is the result of the effects of organic acids, hydrogen peroxide, microbial competition and bacteriocin synthesis [14,16]. Although the bacteriocin action is described in details only as an effect on gram-positive bacteria, the cases of inhibition of gram-negative bacteria are also known in literature [12,13]. This makes lactobacilli especially perspective for plant protection as the majority of phytopathogens are the gram-negative bacteria.In our previous investigation, the use of Lactobacillus plantarum ОNU 87, Lactobacillus plantarum ОNU 206 and Lactobacillus plantarum ОNU 991 strains in plant protection on a model of Kalanchoe daigremontiana Mill. was described. Not only the supernatants with low pH but also the supernatant of L. plantarum ONU 206 culture