No abstract

This study was aimed to evaluate the effect of dietary supplementation of prebiotic (Bio-Mos®, mannoligosacchride), probiotic (Bio-Plus® 2B, Bacillus subtilis and Bacillus licheniformis) and their mixture on growth, biochemical parameters and immune-hematological responses of rabbits. Sixty four New Zealand White male rabbits were divided into 2 equal groups. The 1 st group was uninfected and subdivided into 4 subgroups. The 1 st subgroup fed basal diet (Control), the 2 nd , 3 rd and 4 th subgroups fed on basal diet supplemented with 1 g Bio-Mos, 0.4 g Bio-Plus and 1g Bio-MOS + 0.4 g Bio-Plus / kg, respectively for eight weeks. The 2 nd group was similar to the 1 st group but experimentally infected with Pasteurella multocida. The results in 1 st group showed significant increase (P<0.01) in body weight gain, phagocytic activity (PA), phagocytic index (PI) and total leukocytic counts (TLC) when compared with control group 1.1. In addition, there was significant decrease in serum total cholesterol, triglycerides and glucose when compared with control group 1.1. In 2 nd group , the results showed significant increase (P<0.05) in body weight gain, (P˂0.001) in phagocytic activity and phagocytic index, RBCS count, PCV, Hb concentration, and number of lymphocytes while TLC and number of heterophils showed significant decrease(P<0.001) when compared with control group 2.1 .Also there was significant decrease (P<0.05) in food conversion ratio (FCR), (P<0.01) in total cholesterol and creatinine and (P<0.001) in number of heterophils, triglycerides, glucose, alanine amino transferase (ALT), aspartate amino transferase (AST) and urea in all infected groups fed experimental diets compared with control group 2.1.Supplementing the diet with Bio-Mos, Bio-Plus or their mixture decreased the mortality and improved the adverse clinical signs and post mortem lesions in all experimentally infected groups compared with infected untreated control group. Our results indicate that rabbits received mixture of pre and probiotic groups 1.4 and 2.4 recorded the highest value of daily weight gain, PA, PI, TLC and lymphocytes number and recorded the lowest FCR followed by rabbits received probiotic. Dietary supplementation of prebiotic and probiotic and their mixture improves cell-mediated immune response, liver and kidney functions , decreased the mortality and improved the adverse clinical signs and post mortem lesions in in rabbits experimentally infected with P. multocida.

No abstract

This study was conducted to investigate the effect of dietary supplementation of Aspergillus awamori on aflatoxin B1 (AFB1)-induced liver damage in rabbits. Administration of AFB1 (0.3 mg/kg diet) led to a significant reduction in body weight, body weight gain, total feed intake, total serum proteins, albumin, high density lipoprotein-cholesterol, phagocytic activity, phagocytic index, and the antioxidant enzyme, glutathione peroxidase (GPx). Moreover, AFB1 administration was associated with a significant increase in feed conversion ratio, lipid peroxidation and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase and total bilirubin. In addition, livers of AFB1-supplemented animals showed fatty degeneration with vacuolisation, focal areas of necrosis, mononuclear cells infiltration hyperplasia of bile ducts and sinusoids. A significant increase in the hepatic expression of the biotransformation gene (Cyp3A6), stress-sensitive genes (HO1 and SOD1), and inflammation-related genes (IL6, TNFa, NF-kB, and Cox2) was also observed. Supplementation of the diets with 0.05, 0.1 or 0.15% A. awamori ameliorated all AFB1 deleterious effects with the best improvement observed at the lowest concentration. This is the first investigation to report that supplementation of rabbit diets with A. awamori has an ameliorative effect against AFB1-induced liver damage possibly through preventing hepatic oxidative stress, promoting the antioxidant defence systems, and inhibiting expression of Cyp3A6, HO1, SOD1, IL6, TNFa, NF-kB, and Cox2. Therefore, A. awamori could be used as a potential preventive or therapeutic agent for aflatoxicosis.

No abstract