Abstract. Monosodium glutamate (MSG) is chemical compound that when it is used excessively it can cause various side effects such as hypercholesterolemia. Hypercholesterolemia will result in coronary heart disease (CHD), which is a disease causing death. The aim of study was assessing the effect of almond nut/ Prunus dulcis extract against total cholesterol level in mice that given MSG. This study was randomized post test controlled group design. The sample consisted of 30 mice (Mus musculus L. Strain DDW) that were divided into three groups: control group, treatment group 1 and treatment group 2. The plasma total cholesterol of mice was measured by the method of CHOD with spectrophotometer whose wave length 500nm. Data obtained was analysed by SPSS program version 16. The mean total cholesterol level in mice that treated with Prunus dulcis extraction after 4 weeks experiment was 130.37mg/dl. It were highest compared to the other groups. One way anova showed the significance value was p=0.44), means, there was no difference of total cholesterol level between control and treatment groups 1 and 2. There was no effect of Prunus dulcis extract against total cholesterol level in mice that given monosodium glutamate.