To improve the success rate of microsurgical flap transfers into a buried area, it is important to monitor the circulation of the flap during the early stage. A monitoring flap includes such advantages as simplicity, reliability, noninvasiveness, and the ability to continuously monitor the vascular status of various buried flaps. This article describes experiences related to the importance and reliability of a monitoring flap. A total of 109 flaps in 99 patients were treated with buried free flaps, including a monitoring flap, between 1990 and 1999. Forty-nine patients received a tubed free radial forearm flap with a skin-monitoring flap, and six received a free jejunal flap with a jejunal segment monitoring flap for the reconstruction of the esophagus. Vascularized fibular grafts with a skin monitoring flap or peroneus longus muscle monitoring flap were used for reconstructing the mandible in six patients and for treating osteonecrosis of the femoral head in 48 flaps in 38 patients. Monitoring flap abnormalities were indicated in 14 flaps; therefore, immediate revisions were performed on the pedicle of the monitoring flap and microanastomosis site. Among these 14 flaps, nine showed true thrombosis and five showed false-positive thrombosis. Among the nine flaps that showed true thrombosis, five were salvaged and four were finally lost. The false-positive thrombosis in the five flaps was attributed to torsion or tension of the perforator of the monitoring flap in three flaps, an unclear determination in one flap because the monitoring flap size was too small, and damage to the perforator in the last flap. The total thrombosis rate was 8.3 percent (nine of 109), and the failure rate of the free tissue transfer was 3.7 percent (four of 109). The overall sensitivity of the monitoring flap was 100 percent, the predictive value of a positive test was 64 percent (nine of 14), and false-positive results occurred in 36 percent (five of 14). The salvage rate was 55.6 percent. To improve the reliability of a monitoring flap, it is recommended that the size of the flap be larger than 1 x 2 cm to assess the arterial status, and that a perforator with the appropriate caliber be selected. When a monitoring flap is fixed to a previous incision line or a newly created wound, any torsion or tension of the perforator should be avoided. In conclusion, the current results suggest that a monitoring flap is a simple, extremely useful, and reliable method for assessing the vascular status of a buried free flap.

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The early revascularization of a membranous inlay bone graft in a canine mandible was investigated using bone scans and histological examinations. Eight 5-month-old mongrel dogs were used as the subjects. The inlay bone graft, a 1- x 2-cm critically sized bone, was completely separated from the lower border of the canine mandible and then refixed to the original site. Bone scans and histological examinations were performed in the first, second, third, and fourth postoperative weeks. The bone scan in the first postoperative week revealed radioisotope uptake on the margin of the grafted bone with an isotope count that was 21% of the normal bone uptake. In the second postoperative week, the radioisotope uptake in the grafted bone increased to 52% of the normal bone uptake. In the third and fourth postoperative weeks, the isotope uptakes were 111% and 124%, respectively. The histological findings in the first postoperative week showed an absence of osteoblastic activity and 6 viable blood vessels in a field magnified x 200, which was the equivalent of 25% of the vessels of the normal bone. In the second postoperative week, osteoblastic activity was noted, and the number of viable blood vessels increased to 15, that is, 63% of the vessels of the normal bone. In the third postoperative week, there was an increase in osteoblastic activity, and the number of viable blood vessels was 21, that is, 88% of the vessels of the normal bone. In the fourth postoperative week, there was a marked increase in osteoblastic activity with the number of vessels reaching 23, that is, 96% of the normal bone. In summary, revascularization of the membranous inlay bone graft began within the first week after the bone graft and thereafter gradually increased. In the third postoperative week, revascularization returned to a near-normal value compared with the value of the adjacent normal mandibular bone.

5W, 9W in each group in a random sample of3, application of chloral hydrate intraperitoneal injection anesthesia death, cardiac lavage after removing the damaged spinal cord tissue, immunohistochemical determination of GAP-43. Results: The first, to model after 1D,1W,2W,3W,5W,9W. BBB score, molding 1D after each score is 0points,1W groups of rat hindlimb has recovered, but the score is low, the group had no difference (P>0.05);2W B group, C group, D group was higher than that of A group were significantly different (P<0.05);3W,5W,9W score in group D was higher than that in group B, group C had significant difference (P<0.05). The second ,in the 1W,2W,3W,5W growth to protein GAP-43 immunohistochemical assay,1W group content were increased but no significant difference;2W,3W,5W B group, C group, D group was higher than that of A group were significantly different (P < 0.05), D group higher than that of B group, C group had significant difference (P < 0.01).The third in B group, C group, D group than in A group light complications. Conclusions: The BBB scoreandGAP-43detection, inference of electroacupuncture combined with OECs transplantation in treatment of spinal cord injury in rats at the axon growth there is a synergy, spinal nerve function is greatly improved.