Cell death of retinal pigment epithelium (RPE) is characterized as an essential late-stage phenomenon of dry age-related macular degeneration (AMD). The aim of this study was to elucidate the molecular mechanism underlying RPE cell death after exposure to oxidative stress, which occurs often because of the anatomical location of RPE cells. ARPE-19, an established RPE cell line, exhibited necrotic features involving poly (ADP-ribose) polymerase-1 (PARP-1) activation in response to hydrogen peroxide (H2O2). ARPE-19 cells were resistant to H2O2 when PARP-1 was depleted using siRNA or inhibited by a pharmacological inhibitor of PARP-1, olaparib. Our data suggest a causal relationship between PARP-1 activation and ARPE-19 cell death in response to H2O2. Next, we investigated downstream molecular events in PARP-1 activation. Increased mitochondrial depolarization, mitochondrial fission and alterations of the cellular energy dynamics with reduced NAD+ and ATP were observed in H2O2-treated ARPE-19 cells. H2O2-triggered mitochondrial dysfunction was inhibited by olaparib. Nevertheless, translocation of apoptosis-inducing factor (AIF), a biochemical signature for PARP-1-dependent cell death (parthanatos), was not observed in our study. Moreover, the depletion of AIF did not affect the amplitude of cell death, demonstrating the lack of a role for AIF in the death of ARPE-19 cells in response to H2O2. This feature distinguishes the type of death observed in this study from canonical parthanatos. Next, we examined the in vivo role of PARP-1 in a dry AMD animal model system. Histological analysis of the outer nuclear layer in the mouse retina revealed protection against sodium iodate (SI) following treatment with olaparib. Moreover, retina fundus and electroretinograms also confirmed such a protective effect in the SI-treated rabbit. Collectively, we report that AIF-independent PARP-1-dependent necrosis constitutes a major mechanism of RPE cell death leading to retinal degeneration in dry AMD.

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Recently, yellow poplar (Liriodendron tulipifera L.) is getting attention in Korea due to the fast growing and high yield and quality of lumber. But, it is thought that the color difference between heartwood and sapwood may restrict the practical use of it. This study was aimed to enhance the

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Wood-fiber insulation boards can be utilized as a core construction material. They provide a comfortable and safe residential space and reduce energy consumption because of the ecofriendly nature and high heat insulation. In this study, wood-fiber insulation boards were prepared with different types of adhesive (melamine-urea-formaldehyde (MUF), phenol-formaldehyde (PF), emulsifiable 4,4’ methylene diisocyanate (eMDI), and latex resins), and the physical and heat insulation properties, toxic chemical emissions, and combustion characteristics were analyzed. The different adhesive types had no major effects on the insulation. With regard to the toxic emissions, all wood-fiber insulation boards showed the best rating possible except for the PF resin. In the cone-calorimeter test, the wood-fiber insulation board prepared with MUF showed a lower total heat release, mean heat release rate, smoke release, and CO and CO2 yields than the other samples because of the early formation of the carbonized layer. Based on the comprehensive evaluation, the MUF adhesive is the best choice for wood-fiber insulation boards.