The first milk after birth is also known as colostrum. The composition of animal and human colostrum depends very much on different factors such as: animal species, animal breed and genetical characteristics, feeding program, diet. Bovine colostrum is used in different therapies due to its composition in nutraceuticals, but also because the cow gives high quantity colostrum compared to other animal species (goats or ewes). The immunological properties of colostrum are acknowledged in various studies. Due to its immunoglobiline content, it is an alternative therapy in immunity enhancement. Colostrum is also used in athletes' supplementation plan with very good results in their competition performance. Dry matter composition of cow, goat and ewe's colostrum can give very good information about the nutritional composition, especially proteins. Thus, proteins, lipids, minerals, vitamins are significantly higher in colostrum right after parturition and decrease very much in time in mature milk. Lactose -in most of the studies -presents an increase in concentration from colostrum to milk. Our study reflects the highest content of dry matter in ewe colostrum, with significant decrease in goat and cow. Total lipid content was highest also in ewe's colostrum, lower in cow's, and the lowest in goat colostrum. In most samples, minerals (copper, manganese, zinc) had -the highest concentration in colostrum milking with a decrese from the 6 hour colostrum to the 48 hour colostrum collected after parturition. Iron composition increases in ewe colostrum from the 6 hours to 48 hours.

Living organisms take nutrients from the environment, and together with them, substances with toxic potential � such as heavy metals. Lead is one common metal pollutant especially in aquatic environment, from where the fish can be intoxicated very easily. Bioavailability, distribution, toxic action, synergistic and antagonistic effects are characteristics which can alter the fish health. Our experimental study followed the effects of lead overload in water on iron distribution, in different tissues sample Carassius gibelio Bloch fish. We performed the experiment in four different fish groups: control C; lead � Pb (administration of lead in water 0.075mg/mL of water, as Pb(NO3)2 x � H2O); lead (the same dose) and 2% of freeze-dry garlic incorporated into fishes� food � Pb+garlic; lead (the same dose) and 2% chlorella incorporated into fishes� food � Pb+chlorella, for 21 consecutive days. The iron concentration was analysed with AAS (Atomic Absorption Spectroscopy) from gills, muscle, skin (and scales), intestine, liver, heart, brain, ovary, testicles, and kidney. The obtained data presented a significantly decrease of iron content in all tested tissue samples that demonstrated, alteration of iron homeostasis, explained by a strong antagonistic effect of lead on iron. Our experiment showed that biologic active principles from garlic and chlorella act like natural protectors, and potentiate the iron deficiency even in the case of lead overload in aquatic environment, for fish.

Environmental pollution negatively affects the aquatic ecosystems. Heavy metals are considered dangerous toxic elements for aquatic biota, as metallic elements or as salts which exhibit great stability. Among these, lead is toxic for all aquatic biotic components. Our research investigated the manganese distribution in tissue of Prussian carp fishes after lead intoxication, highlighted also the lead detoxifying potential of active principles from garlic and chlorella (powder). For our experiment 120 Prussian carp fingerlings, weighing 22-25g each, for 21 consecutive days, were divided according to the following treatments: C group (without treatment), E1 group (75ppm Pb in water as Pb(NO3)2 x �H2O), E2 group (75ppm Pb in water+2% freeze-dry garlic in feed), E3 group (75ppm Pb in water+2% freeze-dry chlorella in feed). At the end of the experimental period, tissue samples (gills, muscle myotomers�epaxial, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected after euthanasia with clove oil. Manganese concentration was analytical performed using AAS (atomic absorption spectrometry). Using fish as bioindicators of lead experimental contamination allowed us to obtain valuable informations about its ability to substitut/remove bioactiv minerals from animal tisssues. In the same time, we could emphasize the efficiency of natural antioxidants or chelators of edible plants (such as garlic) or algae (such as chlorella), to alleviat the lead impact on homeostasis of trace elements from tissues.

In living organisms lead is classified as potential toxic metal, and in high concentration can produce intoxication with the alteration of some vital organs, especially liver and kidney. In aquatic environment lead can be absorbed by fishes and other organisms, with different distribution in various tissues. Our aim of experiment was to verify and demonstrate the protective effect of lyophilized garlic and chlorella against bioaccumulation of lead in fishes living in aquatic environment deliberated polluted with lead. Thus, lyophilized garlic and chlorella administrated as supplements in fodder for fishes (Carassius gibelio) diminished the antagonistic effect of lead against zinc in all tested tissues: liver, kidney, heart, brain, ovary, testis, muscles myotome -epaxial, skin -with scales, gills, and intestine.

Romanian Mioritic Shepherd dog was selected from a natural population breed in Carpathian Mountains. The aim of this paper was to estimate the correlation coefficients between 6 characteristics, based on individual values from 23 females from Romanian Mioritic Shepherd dog breed. The animals were registered in the Romanian Mioritic Association Club from Romania. Following the analysis of the obtained phenotypic correlation coefficients, we concluded that between the height at withers and length of the body; height at withers and length of the head; length of the head and length of the skull, the correlations are strongly positive and between the other characters taken into study the correlations are average and weakly positive or weakly negative.